02520nas a2200217 4500008004100000245011500041210006900156260001600225300001200241490000600253520178000259653001602039653002102055653002302076653002502099653002602124100002902150700002402179700003102203856006802234 2016 eng d00aIn vivo Study on Depressant Effects and Muscle Coordination Activity of Galphimia glauca Stem Methanol Extract0 aIn vivo Study on Depressant Effects and Muscle Coordination Acti cAugust 2016 a219-2250 v83 a

Background: Galphimia glauca is an evergreen shrub found across peninsular India, belonging to family Malpighiaceae. Objective: The objective of this study was to assess the in vivo depressant effects and muscle coordination activity of G. glauca stem methanol extract (GGSME). Materials and Methods: The stem methanol extract was administered in Swiss albino mice in 1 day to study the central nervous system (CNS) depressant and muscle coordination activity employing animal models such as sodium pentobarbital‑induced sleep test, hole‑board test, open field test, pentylenetetrazole (PTZ)‑induced convulsions, picrotoxin‑induced convulsions, grip strengthening test in mice, and Rota‑rod test. Results: The LD50 of GGSME was found to be >2000 mg/kg body weight (b.w.). Mice treated with stem methanol extract at 100, 200, and 400 mg/kg, b.w. doses extended the sleeping time induced by sodium pentobarbital (40 mg/kg. b.w., i.p.). The stem methanol extract at 400 mg/kg dose showed a significant (P ≤ 0.001) dose‑dependent decrease in the number of rears and head dipping number in the hole‑board test. The extract exhibited a significant (P ≤ 0.001) effect on the ambulatory behavior of mice in the open field test and also extended the onset of seizures induced by PTZ (90 mg/kg b.w., i.p.) and picrotoxin (10 mg/kg, b.w., i.p.). The extract also exhibited significant (P ≤ 0.001) effects on muscle coordination in rota‑rod and grip strengthening test in mice. Conclusion: The study results conclude that the GGSME has a potential CNS depressant and muscle relaxant effects compared to the standard drugs.

10aConvulsions10aGalphimia glauca10aGrip strength test10aSodium pentobarbital10aStem methanol extract1 aGarige, Baba, Shankar Ra1 aKeshetti, Srisailam1 aVattikuti, Uma, Maheshwara uhttps://www.phcogres.com/article/2016/8/4/1041030974-8490188878