02521nas a2200289 4500008004100000245017300041210006900214260001300283300001200296490000600308520160000314653001201914653000901926653001701935653001501952653001901967653001101986100001601997700001802013700001502031700002702046700002002073700001702093700001602110700003702126856006802163 2016 eng d00aInhibition of Group IIA Secretory Phospholipase A2 and its Inflammatory Reactions in Mice by Ethanolic Extract of Andrographis paniculata, a Well‑known Medicinal Food0 aInhibition of Group IIA Secretory Phospholipase A2 and its Infla cMay 2016 a213-2160 v83 a
Andrographis paniculata Nees is an important medicinal plant found in the tropical regions of the world, which has been traditionally used in Indian and Chinese medicinal systems. It is also used as medicinal food. A. paniculata is found to exhibit anti‑inflammatory activities; however, its inhibitory potential on inflammatory Group IIA phospholipases A2 (PLA2) and its associated inflammatory reactions are not clearly understood. The aim of the present study is to evaluate the inhibitory/neutralizing potential of ethanolic extract of A. paniculata on the isolated inflammatory PLA2 (VRV‑PL‑VIIIa) from Daboii rusellii pulchella (belonging to Group IIA inflammatory secretory PLA2 [sPLA2]) and its associated edema‑induced activities in Swiss albino mice. A. paniculata extract dose dependently inhibited the Group IIA sPLA2 enzymatic activity with an IC50 value of 10.3 ± 0.5 μg/ml. Further, the extract dose dependently inhibited the edema formation, when co‑injected with enzyme indicating that a strong correlation exists between lipolytic and pro‑inflammatory activities of the enzyme. In conclusion, results of this study shows that the ethanolic extract of A. paniculata effectively inhibits Group IIA sPLA2 and its associated inflammatory activities, which substantiate its anti‑inflammatory properties. The results of the present study warranted further studies to develop bioactive compound (s) in ethanolic extract of A. paniculata as potent therapeutic agent (s) for inflammatory diseases.
10aDisease10aDrug10aInflammation10aInhibition10aNeutralization10aPlants1 aKishore, V.1 aYarla, N., S.1 aZameer, F.1 aPrasad, M., N. Nagendr1 aSantosh, M., S.1 aMore, S., S.1 aRao, D., G.1 aDhananjaya, Bhadrapura, Lakkappa uhttps://www.phcogres.com/article/2016/8/3/1041030974-8490182916