02403nas a2200229 4500008004100000245012000041210006900161260001800230300001200248490000600260520164400266653000601910653004601916653001601962653001701978653002801995653002702023100001602050700001802066700002102084856006802105 2016 eng d00aEvaluation of In Vitro Cytotoxic and Antioxidant Activity of Datura metel Linn. and Cynodon dactylon Linn. Extracts0 aEvaluation of In Vitro Cytotoxic and Antioxidant Activity of Dat cFebruary 2016 a123-1270 v83 a
Aim: To evaluate in vitro cytotoxicity and antioxidant activity of Datura metel L. and Cynodon dactylon L. extracts. Materials and Methods: The extraction of plants parts (datura seed and fruit pulp) and areal parts of durva was carried out using soxhlet and cold extraction method using solvents namely methanol and distilled water. The total phenolic content (TPC) and total flavonoid content (TFC) was determined by established methods. The in vitro cytotoxicity assay was performed in vero cell line by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay method. In vitro antioxidant activity of the extract was performed by 2, 2‑diphenyl‑1‑picrylhydrazyl radical scavenging method. Results: We found that the highest amount of TPC and TFC in methanolic extracts of seed (268.6 μg of gallic acid equivalence/mg of dry plant material) and fruit pulp (8.84 μg of quercetin equivalence/mg dry plant material) of D. metel, respectively prepared by Soxhlet method. The methanolic extract of C. dactylon prepared using soxhlation has shown potent free radical scavenging activity with 50% inhibitory concentration (IC50) value of 100 μg/ ml. The IC50 of a methanolic cold extract of datura fruit was found to be 3 mg/ml against vero cell line. Conclusion: We observed that plant parts of C. dactylon and D. metel have a high antioxidant activity. Further research is needed to explore the therapeutic potential of these plant extracts.
10a210a2‑diphenyl‑1‑picrylhydrazyl radical10aAntioxidant10acytotoxicity10aTotal flavonoid content10aTotal phenolic content1 aRoy, Soumen1 aPawar, Sandip1 aChowdhary, Abhay uhttps://www.phcogres.com/article/2016/8/2/1041030974-8490175610