02599nas a2200253 4500008004100000245010000041210006900141260001500210300001000225490000600235520178000241653001302021653002702034653002102061653002502082653001002107100002202117700003902139700001902178700003002197700002202227700002802249856006802277 2016 eng d00aExtracts from Flammulina velutipes Inhibit the Adhesion of Pathogenic Fungi to Epithelial Cells0 aExtracts from Flammulina velutipes Inhibit the Adhesion of Patho cMarch 2016 a56-600 v83 a

Background: Recently, extracts from natural sources have been tested for their antifungal properties. In this aspect, Flammulina velutipes extracts possess a significant amount of branch‑chained carbohydrates with mannose moieties that, hypothetically, can reduce the adhesion. Objective: In this study, we assessed the capacity of extracts from F. velutipes (wild‑type AQF‑1 and ATCC 34574 as the reference strain) to inhibit the adhesion of S. schenkii and C. albicans to epithelial cells. Materials and Methods: The aqueous extracts from F. velutipes strains were obtained by sonication, total carbohydrate and protein was analyzed by Dubois and Lowry methods respectively. Effect of the extracts (50, 100 and 150 μg/mL) on the fungi adhesion to host cells was evaluated after 1 h interaction, and the percentage of inhibition of adhesion was measured. After of interaction the cytoskeleton from cell was analyzed with phalloidin‑FITC. Results: The extract from strain AQF‑1 (50, 100 and 150 μg/mL) inhibited the adhesion of: S. schenkii in a dose‑dependent manner (4.9, 7.5 and 12.7%, respectively) and C. albicans in a dose‑independent manner (5.2%). The percentage of inhibition by extracts from the strain ATCC34574 at the same concentrations, shown that are dose independent for both fungi: 3.9% for S. schenkii and 2.6% for C. albicans. Conclusion: The extracts from F. velutipes inhibit the adhesion of pathogenic fungi to host cells. The mechanism molecular is unknown; however, is probably an interaction between the polysaccharides from extracts with the fungi receptors. This aspect is currently analyzed.

10aAdhesion10abasidiomycets extracts10aepithelial cells10aFlammulina velutipes10afungi1 aKashina, Svetlana1 aVillavicencio, Lérida, Liss Flore1 aBalleza, Marco1 aSabanero, Gloria, Barbosa1 aTsutsumi, Víctor1 aLópez, Myrna, Sabanero uhttps://www.phcogres.com/article/2016/8/5/1041030974-8490178648