02994nas a2200337 4500008004100000245011900041210007100160260001500231300001000246490000600256520190600262653001602168653002402184653001402208653001402222653001502236653001402251100002702265700003402292700002802326700002402354700002302378700003502401700003302436700002302469700003102492700002002523700001902543700002602562856006802588 2016 eng d00aAnti‑inflammatory Activity of Berry Fruits in Mice Model of Inflammation is Based on Oxidative Stress Modulation0 aAnti‑inflammatory Activity of Berry Fruits in Mice Model of Infl cMarch 2016 a42-490 v83 a

Background: Many fruits have been used as nutraceuticals because the presence of bioactive molecules that play biological activities. Objective: The present study was designed to compare the anti‑inflammatory and antioxidant effects of methanolic extracts of Lycium barbarum (GOJI), Vaccinium macrocarpon (CRAN) and Vaccinium myrtillus (BLUE). Materials and Methods: Mices were treated with extracts (50 and 200 mg/kg, p.o.), twice a day through 10 days. Phytochemical analysis was performed by high‑performance liquid chromatography. Antioxidant activity was determine by 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) assay, reducing power, lipid peroxidation thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and catalase (CAT) activity. Anti‑inflammatory activity was evaluated by paw edema followed by determination of myeloperoxidase (MPO) and TBARS. Results: High amount of phenolic compounds, including rutin, were identified in all berries extracts. However, quercetin was observed only in BLUE and CRAN. GOJI presents higher scavenging activity of DPPH radical and reducing power than BLUE and CRAN. The extracts improved antioxidant status in liver; BLUE showed the largest reduction (75.3%) in TBARS when compared to CRAN (70.7%) and GOJI (65.3%). Nonetheless, CAT activity was lower in BLUE group. However, hepatic concentrations of GSH were higher in animals treated with GOJI rather than CRAN and BLUE. Despite all fruits caused a remarkable reduction in paw edema and TBARS, only BLUE and CRAN were able to reduce MPO. Conclusion: These results suggest that quercetin, rutin, or other phenolic compound found in these berry fruits extracts could produce an anti‑inflammatory response based on modulation of oxidative stress in paw edema model.

10aAntioxidant10aAnti‑inflammatory10ablueberry10acranberry10aGoji berry10aPaw edema1 aNardi, Geisson, Marcos1 aJanuario, Adriana, Graziele F1 aFreire, Cassio, Geremia1 aMegiolaro, Fernanda1 aSchneider, Kétlin1 aPerazzoli, Marlene, Raimunda A1 aNascimento, Scheley, Raap Do1 aGon, Ana, Cristina1 aMariano, Luísa, Nathália1 aWagner, Glauber1 aNiero, Rivaldo1 aLocatelli, Claudriana uhttps://www.phcogres.com/article/2016/8/5/1041030974-8490178642