03277nas a2200325 4500008004100000245014900041210006900190260001400259300001200273490000600285520223500291653002402526653001602550653002202566653002102588653002202609653001802631653002302649653001102672653002202683653001802705100002202723700002502745700002402770700002402794700002202818700002302840700002002863856006802883 2015 eng d00aStructural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by gas chromatography: Mass spectroscopy0 aStructural elucidation of chemical constituents from Benincasa h cJune,2015 a282-2930 v73 a

Background: Benincasa hispida (BH) and Carissa congesta (CC) are regarded as ethnopharmacological imperative plants in Asian countries. Objectives: Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in the BH and CC extracts. The presence of lupeol has been reported previously by us using high‑performance thin‑layer chromatography and high‑performance liquid chromatography. Materials and Methods: Present research studies encompasses identification of chemical constituents in BH seeds and CC roots petroleum ether extracts by hyphenated technique such as gas chromatography‑mass spectroscopy (MS) which when coupled gives a clear insight of constituents. Results: The components were identified by matching mass spectra with MS libraries. There were 13 and 10 different compounds analyzed from CC and BH, respectively. The components present were Pentanoic acid, 5‑hydroxy, 2,4‑butylphenyl; n‑Hexadecanoic acid (Palmitic acid); Sulfurous acid, 2‑ethylhexylhepatdecyl ester; n‑Tridecane; 6‑methyltridecane; (9E, 12E)‑9,12‑Octadecadienyl chloride, Hexadecanoic acid, 3‑(trimethylsilyl)‑oxy] propyl ester; 9,12‑Octadecadenoic acid, 2 hydroxy‑1‑(hyroxymethylethyl) ester; 9,12‑Octadecadienoic acid, 2,3 dihydroxypropyl ester; n‑Propyl‑9,12‑Octadecadienoate, Lupeol; Taraxasterol; 6a, 14a‑Methanopicene, perhydro‑12,4a, 61a, 9,9,12a‑hepatmethyl‑10‑hydoxy and 9‑Octadecene; 2‑Isoprpenyl‑5‑methyl‑6‑hepten‑1‑ol; n‑Hexadecanoic acid, 2‑hyroxy‑1‑(hydroxymethyl) ethyl ether; Butyl‑9,12‑Octadecadieonate; Friedoolean‑8‑en‑3‑one; friedours‑7‑en‑3‑one; 13,27‑Cyclosuran‑3‑one; Stigmaste‑7,25‑dien‑3‑ol (3β, 5α); Stigmasta‑7,16‑dien‑3‑ol; chrondrillasterol in BH seeds and CC roots extracts respectively. Conclusion: Eluted components from the extracts could provide further researchers to work with various pharmacological activities related models and studies.

10a25‑dien‑3‑ol.10aApocynaceae10aBenincasa hispida10aCarissa congesta10aChrondrillasterol10aCururbitaceae10aGas chromatography10aLupeol10aMass spectroscopy10aStigmaste‑71 aDoshi, Gaurav, M.1 aNalawade1, Vivek, V.1 aMukadam, Aaditi, S.1 aChaskar, Pratip, K.1 aZine, Sandeep, P.1 aSomani, Rakesh, R.1 aUne, Hemant, D. uhttps://www.phcogres.com/article/2015/7/3/1041030974-8490157179