02669nas a2200277 4500008004100000245012400041210006900165260001900234300001200253490000600265520180800271653000602079653003802085653001602123653002402139653001402163653001402177653001802191100002002209700003002229700001602259700001802275700001702293700002002310856006102330 2017 eng d00aAntioxidant Activity and Phenolic and Flavonoid Contents of the Extract and Subfractions of Euphorbia splendida Mobayen0 aAntioxidant Activity and Phenolic and Flavonoid Contents of the  cNovember 2017 a362-3650 v93 a

Introduction: The harmful action of the free radicals which cause the oxidative stress can be blocked by antioxidant substances, and different plant extracts showed antioxidant activity. The aim of this study is was evaluation the antioxidant activity of total methanol extract (ME) and subfractions of Euphorbia splendida Mobayen. Materials and Methods: Aerial part of E. splendida was extracted by maceration with methanol and then subfractionated by liquid–liquid fractionation using petroleum ether, chloroform, ethyl acetate, and water. Antioxidant activity was assessed by 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) radical scavenging activity assay, reduction of ferric ions and ferrous ion chelating potential. Total phenolic contents (TPC) and total flavonoid contents (TFC) were estimated with Folin‑Ciocaltue and aluminum chloride methods, respectively. Results: The findings revealed that E. splendida ME and subfractions showed a dose‑dependent antioxidant activity. ME showed the highest antioxidant activity based on total reduction capability and ferrous ions chelating assay tests. Aqueous fraction and then ethyl acetate fraction showed the best IC50 in DPPH radical scavenging test in comparison to butylated hydroxytoluene. ME showed the highest value of TPC and TFC (270.74 ± 0.005 mg/g and 208.23 ± 0.007 mg/g, respectively). Conclusion: This study showed that the extract and subfractions of E. splendida have antioxidant activity. The antioxidant activity of the extract and fractions might be attributed to the presence of phenolic compounds. More studies are needed to determine the active antioxidant compounds of this plant.

10a110a1‑Diphenyl‑1‑picrylhydrazyl10aAntioxidant10aEuphorbia splendida10aFlavonoid10aFractions10aFree radicals1 aKefayati, Zahra1 aMotamed, Saeed, Mohammadi1 aShoja, Asie1 aNoorix, Mitra1 aNoori, Mitra1 aGhods, Roshanak uhttps://www.phcogres.com/article/2017/9/4/104103prpr1217