02743nas a2200265 4500008004100000245009300041210006900134260001900203300001200222490000600234520189800240653002302138653001502161653002202176653003202198653001902230653002602249653002002275100001502295700001802310700002402328700003302352700003102385856006102416 2017 eng d00aInhibitory Effects of Pterodon emarginatus Bean Oil and Extract on Staphylococcus aureus0 aInhibitory Effects of Pterodon emarginatus Bean Oil and Extract  cNovember 2017 a348-3530 v93 a

Background: Pterodon emarginatus is a tree of the Brazilian Savannah. The beans of this tree are used in folk medicine as anti‑inflammatory preparations, especially for infections caused by Staphylococcus aureus. These bacteria can cause simple infections or serious illnesses such as pneumonia, meningitis, endocarditis, toxic shock syndrome, septicemia, and others. Objective: This study had the goal of verifying the effect of the essential oil (OE) from P. emarginatus on the inhibition of S. aureus in culture medium, i.e., “in vitro” tests. Materials and Methods: The vegetable material was cut and crushed with a press. The OE was obtained by extraction using hexane, alcohol, and water. The P. emarginatus extracts obtained were used to evaluate the antimicrobial effect on S. aureus (ATCC 25923) by tests of well diffusion, disc diffusion, and microdilution. The strain used in the assays was maintained in brain heart infusion broth and nutrient agar until testing. Afterward, the bacteria were spread on agar plates with Mueller‑Hinton agar medium. In the wells and on the paper discs, the OE suspensions were placed in the following volumes: 10, 15, 20, 25, 30, 40, and 80 µL and subsequently they were incubated at 35°C ± 2°C. After 24 h, the number of colony‑forming unit was determined. Results: Pure OE and hydroalcoholic extract inhibited the growth of S. aureus, while aqueous extract had no effect on bacterial growth in all microbial methods used. Conclusion: Thus, the present study showed the potential of sucupira‑based extracts against S. aureus growth, opening new perspectives for the evaluation of these bioactive compounds as phytopharmaceutical products.

10aAntimicrobial test10aDiterpenes10amedical pathogens10aPhytopharmaceutical extract10aSesquiterpenes10aStaphylococcus aureus10aSucupira-branca1 aMendes, VS1 aSant'Anna, JB1 aOliveira, S., C. C.1 aMaldonade, Iriani, Rodrigues1 aMachado, Eleuza, Rodrigues uhttps://www.phcogres.com/article/2017/9/4/104103prpr1317