02806nas a2200241 4500008004100000245018400041210006900225260001400294300001200308490000600320520199300326653001302319653002702332653002302359653001102382653002002393653001402413100001502427700001802442700001902460700001702479856006802496 2015 eng d00aPsidium guajava Linn. Leaf Extract Affects Hepatic Glucose Transporter‑2 to Attenuate Early Onset of Insulin Resistance Consequent to High Fructose intake: An Experimental Study0 aPsidium guajava Linn Leaf Extract Affects Hepatic Glucose Transp cJune 2015 a166-1750 v73 a

Background: Insulin resistance (IR) is amalgam of pathologies like altered glucos metabolism, dyslipidemia, impaired glucose tolerance, non-alcoholic fatty liver disease, and associated with type-II diabetes and cardiometabolic diseases. One of the reasons leading to its increased and early incidence is understood to be a high intake of processed fructose containing foods and beverages by individuals, especially, during critical developmental years. Objective: To investigate the preventive potential of aqueous extract of Psidium guajava leaves (PG) against metabolic pathologies, vis-à-vis, IR, dyslipidemia, hyperleptinemia and hypertension, due to excess fructose intake initiated during developmental years. Materials and Methods: Post-weaning (4 weeks old) male rats were provided fructose (15%) as drinking solution, ad libitum, for 8 weeks and assessed for food and water/fructose intake, body weight, fasting blood sugar, mean arterial pressure, lipid biochemistry, endocrinal (insulin, leptin), histopathological (fatty liver) and immunohistochemical (hepatic glucose transporter [GLUT2]) parameters. Parallel treatment groups were administered PG in doses of 250 and 500 mg/kg/d, po × 8 weeks and assessed for same parameters. Using extensive liquid chromatography-mass spectrometry protocols, PG was analyzed for the presence of phytoconstituents like Myrecetin, Luteolin, Kaempferol and Guavanoic acid and validated to contain Quercetin up to 9.9%w/w. Results: High fructose intake raised circulating levels of insulin and leptin and hepatic GLUT2 expression to promote IR, dyslipidemia, and hypertension that were favorably re-set with PG. Although PG is known for its beneficial role in diabetes mellitus, for the first time we report its potential in the management of lifelong pathologies arising from high fructose intake initiated during developmental years.

10aFructose10aGlucose Ttransporter-210aInsulin resistance10aLeptin10aPsidium guajava10aQuercetin1 aMathur, R.1 aDutta, Shagun1 aVelpandian, T.1 aMathur, S.R. uhttps://www.phcogres.com/article/2015/7/2/1041030974-8490151459