02003nas a2200241 4500008004100000245004200041210004200083260001500125300000800140490000600148520130500154653002801459653002101487653001901508653001201527100003101539700002801570700002701598700001801625700002101643700002801664856006901692 2016 eng d00aAnti‑Leishmania Activity of Osthole0 aAnti‑Leishmania Activity of Osthole cMarch 2016 a1-40 v83 a

Background: Treatment of cutaneous leishmaniasis (CL) is occasionally highly resistant to pentavalent antimonials, the gold standard in pharmacotherapy of CL. Since there is no effective vaccine, the discovery of natural antileishmanial products as complementary therapeutic agents could be used to improve the current regimens. Objective: In this study in vitro and in vivo antileishmanial activities of osthole, a natural coumarin known to possess antibacterial and parasiticidal activities are evaluated. Materials and Methods: Leishmania major infected J774.A1 macrophages were treated with increasing concentrations of osthole. CL lesions of BALB/c mice were treated topically with 0.2% osthole. Results: Osthole exhibited dose‑dependent leishmanicidal activity against intracellular amastigotes with IC50 value of 14.95 μg/ml. Treatment of CL lesions in BALB/c mice with osthole significantly declined lesion progression compared to untreated mice (P < 0.05), however did not result in recovery. Conclusion: Osthole demonstrated remarkable leishmanicidal activity in vitro. Higher concentrations of osthole may demonstrate the therapeutic property in vivo.

10aCutaneous leishmaniasis10aLeishmania major10aLeishmanicidal10aOsthole1 aKermani, Elaheh, Kordzadeh1 aSajjadi, Seyed, Ebrahim1 aHejazi, Seyed, Hossein1 aArjmand, Reza1 aSaberi, Sedigheh1 aEskandarian, Abbas, Ali uhttps://www.phcogres.com/article/2016/8/1s/1041030974-8490178650