02581nas a2200205 4500008004100000245009000041210006900131260001400200300001200214490000600226520192000232653001802152653002802170653003502198653001102233100002202244700002102266700002002287856006802307 2017 eng d00aEvaluation of anti-inflammatory and antimicrobial activity of AHPL/AYCAP/0413 capsule0 aEvaluation of antiinflammatory and antimicrobial activity of AHP cJuly 2017 a273-2760 v93 a

Background: Conventional therapeutic agents used for treatment of Acne are associated with various adverse effects necessitating development of safe and effective alternative therapeutic agents. In this context, a polyherbal formulation AHPL/AYCAP/0413 was developed for treatment of Acne. Objectives: To evaluate Anti-inflammatory and antimicrobial activity of AHPL/AYCAP/0413. Material and Methods: 1) Anti-inflammatory activity: Anti-inflammatory activity of AHPL/AYCAP/0413 in comparison with Diclofenac was assessed in carrageenan induced rat Paw edema model. 2) Anti-microbial activity for P. acne: Propionibacterium acnes were incubated under anaerobic conditions. Aliquots of molten BHI with glucose agar were used as the agar base. Formulation and clindamycin (10 μg/ml) were introduced in to the Agar wells randomly. 3) Anti-microbial activity for Staphylococcus epidermidis and Staphylococcus aureus: Staphylococcus epidermidis and Staphylococcus aureus were incubated under aerobic conditions at 37°C. TSB with glucose agar was used as the agar base. 0.5ml of formulation and clindamycin (10 μg/ml) were introduced in to the wells randomly. The antibacterial activity was evaluated by measuring zones of inhibition (in mm). Result: Significant reduction in rat paw edema (51% inhibition) was observed with formulation AHPL/AYCAP/0413 which was also comparable to that of Diclofenac (58% inhibition). Zone of inhibition for formulation was 18.33 mm, 19.20 mm and 26.30 mm for P. acnes, S. epidermidis and S. aureus respectively. This activity was also comparable to that of Clindamycin. Conclusion: AHPL/AYCAP/0413 capsule possesses significant Anti-inflammatory and Anti-microbial activities which further justifies its role in the management of Acne vulgaris.

10aAcne vulgaris10aAHPL/AYCAP/0413 capsule10aAlternative system of medicine10aHerbal1 aNipanikar, Sanjay1 aChitlange, Sohan1 aNagore, Dheeraj uhttps://www.phcogres.com/article/2017/9/3/1041030974-8490210328