02363nas a2200265 4500008004100000245009900041210006900140260001800209300001000227490000600237520151500243653000901758653003601767653004201803653002101845653002601866653002001892653001401912653003101926100001601957700001501973700002601988700001502014856006802029 2016 eng d00aIsolation and Characterization of Chemopreventive Agent from Sphaeranthus amaranthoides Burm F0 aIsolation and Characterization of Chemopreventive Agent from Sph cDecember 2015 a61-650 v83 a

Objective: To investigate the in vitro cytotoxic effect and to isolate and characterize a chemopreventive secondary metabolite from Sphaeranthus amaranthoides Burm F (sivakaranthai). Materials and Methods: In vitro cytotoxic effect was carried out by 3 (4,5‑dimethylthiazol‑2‑yl) 2,5‑diphenyltetrazolium bromide assay. Different concentrations of the extracts were tested on three different cell lines namely A549, HT29, and MCF7. The chloroform extract was subjected to column chromatography, and the isolated compound was characterized by various spectral methods and by single crystal X‑ray crystallography. Results: The concentration that cause 50% growth inhibition value of chloroform extract was found to be 0.9 and 19 μg/mL against MCF7 and A549 cell lines, respectively. Chloroform extract was subjected to column chromatography for the isolation of phytoconstituent. The structure of the isolated compound was identified by spectroscopic techniques such as infrared, nuclear magnetic resonance, XRD, and mass spectroscopy. On comparison of complete spectral detail of the compound, the proposed structure was identified as chrysosplenol D (a flavonoid). Chrysosplenol D was isolated for the first time from this plant. Conclusion: The chloroform extract had higher cytotoxic effect, and the isolated chrysosplenol D may be responsible for the anti‑proliferative effect of the plant.

10a3 (410a5‑dimethylthiazol‑2‑yl) 210a5‑diphenyltetrazolium bromide assay10aCharacterization10aChemopreventive agent10aChrysosplenol D10aIsolation10aSphaeranthus amaranthoides1 aGayatri, S.1 aSuresh, R.1 aReddy, Uma, Maheswara1 aChitra, K. uhttps://www.phcogres.com/article/2016/8/1/1041030974-8490171101