02300nas a2200205 4500008004100000245012800041210006900169260001800238300001000256490000600266520159000272653001601862653002101878653002301899653001801922653002101940100002801961700003701989856006802026 2016 eng d00aAntioxidant and Immunomodulatory Activity of Hydroalcoholic Extract and its Fractions of Leaves of Ficus benghalensis Linn.0 aAntioxidant and Immunomodulatory Activity of Hydroalcoholic Extr cDecember 2015 a50-550 v83 a

Background: Ficus benghalensis is a folk medicine indigenous plant of India. Several studies on this plant reported and focused on the biological profile of the plant. Objectives: This study is aimed to evaluate the antioxidant and immunomodulatory activity of F. benghalensis leaf extract using various in vitro screening methods of both parameters. Materials and Methods: Hydroalcoholic (FB1) extract and it’s four fractions viz. n‑hexane (FB2), n‑butanol (FB3), chloroform (FB4), and water (FB5) of leaves of F. benghalensis investigated for their free radical scavenging activity using 1‑1‑diphneyl‑2‑picrylhydrazyl and 2, 2’‑azino‑bis (3‑ethylbenzthiazoline‑6‑sulfonic acid) radicals. A dose‑response curve was plotted and IC50 values were determined to assess antioxidant activity. Nitroblue tetrazolium test, phagocytosis of killed Candida albicans and candidacidal assay were carried out to assess the immunomodulatory activity. Positive non‑lymphoid cell number, mean particle number of killed C. albicans, percent value of killed C. albicans by neutrophils were calculated and presented. Results: All extracts showed antioxidant and prominent immunomodulatory activity with compared to standard. Conclusions: Hydroalcoholic (FB1) extract and its four fractions viz. n‑hexane (FB2), n‑butanol (FB3), chloroform (FB4), and water (FB5) showed promising antioxidant and immunomodulatory activity.

10aAntioxidant10aCandida albicans10aFicus benghalensis10aFree radicals10aImmunomodulatory1 aBhanwase, Anil, Subhash1 aAlagawadi, Kallanagouda, Ramappa uhttps://www.phcogres.com/article/2016/8/1/1041030974-8490171107