03492nas a2200229 4500008004100000245014300041210006900184260001800253300001000271490000600281520263800287653000602925653008202931653002503013653002103038653002303059653002103082653003103103100003103134700002903165856006803194 2016 eng d00aMethanolic Extract of Curcuma caesia Roxb. Prevents the Toxicity Caused by Cyclophosphamide to Bone Marrow Cells, Liver and Kidney of Mice0 aMethanolic Extract of Curcuma caesia Roxb Prevents the Toxicity  cDecember 2015 a43-490 v83 a

Introduction: With an ever increasing cause of cancer, it has been recommended to treat with conventional drugs, however because of the side effects caused by the conventional drugs, the research on medicinal plants has been intensified due to their less adverse and toxic effects. Objectives: The primary objective of the present study was to evaluate the protective effect of the medicinal plant Curcuma caesia Roxb. against free radicals ABTS+ and O2 -. Also it was aimed to evaluate the protective effect of C.caesia Roxb. against the chemotherapeutic drug Cyclophosphamide and its side effects in liver and kidney. Methods: The rhizomes of the plant was extracted with methanol through soxhlet and its antioxidant activity was tested against ABTS+ and O2 - . For antigenotoxic studies, animals were divided into eight groups and micronucleus assay was employed and for biochemical analysis serum sample was collected from the blood and SGOT, SGPT analysis was performed. Also the biochemical analysis was performed from both the liver and kidney. Results: The methanolic extract of Curcuma caesia Roxb. was found to scavenge the free radicals ABTS+ and O2 - . the micronuclei formation was found to be increased in the positive control group as compared to the negative control group significantly (P<0.002) however increase in the number of micronuclei was found to be decrease with the pretreatment of the extract at different concentrations significantly as compared to the negative control groups (P<0.01, P<0.005,P<0.001). The increased level of serum SGPT and SGOT as well as peroxidation level in both liver and kidney due to treatment of cyclophosphamide was also found to be decreased with the pretreatment of the extract significantly as compared to the positive control groups. There was decreased in the level of endogenous antioxidant such as GSH and GR in the positive control group however decreased level of GSH and GR was found to be increased with the pretreatment of the methanolic extract of C. caesia Roxb. Conclusion: The present study suggested that the methanolic extract of C. caesia Roxb has not shown any genotoxicity and reduces the genotoxicity caused by cyclophosphamide. It was also to have the protective effects against the liver and kidney. So it could be provided as one of the herbal supplementation in chemoprevention of CP to ameliorate the side effects of it.

10a210a2’ azino bis (ethylbenzthiazolene‑6‑sulfonic acid) radical cation assay10aCurcuma caesia Roxb.10aCyclophosphamide10amicronucleus assay10aOxidative stress10aSuperoxide dismutase assay1 aDevi, Heisanam, Pushparani1 aMazumder, Pranab, Behari uhttps://www.phcogres.com/article/2016/8/1/1041030974-8490171106