02965nas a2200265 4500008004100000245009200041210006900133260001500202300001200217490000600229520206100235653005002296653006202346653002402408653002002432653002002452100001702472700002302489700001802512700002502530700002302555700003202578700002102610856006802631 2017 eng d00aBotanical and Chemical Fingerprinting of Medicinal Roots of Justicia gendarussa Burm f.0 aBotanical and Chemical Fingerprinting of Medicinal Roots of Just cApril 2017 a208-2140 v93 a

Background: Justicia gendarussa Burm f. of family Acanthaceae is medicinally important herb used in the treatment of inflammatory disorders, asthma, hepatic injuries, pathogenic infection and also shows antiproliferative activity against various cancer cell lines. Materials and Methods: Pharmacognostical evaluation (macro-microscopy, physicochemical analysis and preliminary phytochemical analysis), high-performance thin layer chromatography (HPTLC) fingerprinting and chemical profiling by gas chromatography-mass spectrometry (GCMS) of dried roots of J. gendarussa were done according to quality standard procedures. Results: Microscopic analysis revealed the compact arrangement of cells in cork region and thin-walled cortex beneath epidermis. Parenchymatous cells with xylem vessel were observed in the roots of J. gendarussa. Physicochemical studies revealed loss on drying (10.474%), total ash (2.990%), acid-insoluble ash (0.099%), water-soluble ash (1.528%), alcohol-soluble extractive value (0.564%) and water-soluble extractive value (4.11%) of the raw drug. Preliminary phytochemical analysis of ethanolic extract of J. gendarussa showed the presence of alkaloid, steroid, flavonoid, phenol, carbohydrate, saponin and quinone. Rf, color of the spots and densitometric scan were recorded by HPTLC fingerprinting using toluene: ethyl acetate: formic acid (5.0:4.0:1.0). On photodocumentation, six spots were visualized under 254 nm, nine spots under 360 nm and six spots at 620 nm. Identification of components in ethanolic extract of J. gendarussa was done by GC-MS. GC-MS results in the presence of oleic acid, 9,12-octadecadienoic acid, 6,9,12-octadecatrienoic acid and estra-1, 3,5 (10)-trein-17-β-ol in ethanolic extract of J. gendarussa. Conclusion: These specific identities will be useful in identification and authentication of the raw drug in dried form.

10aGas chromatography-mass specrtometry analysis10aHigh-performance thin layer chromatography fingerprinting10aJusticia gendarussa10aPharmacognostic10aQuality control1 aYadav, Deepa1 aReshi, Mohd, Salim1 aUthra, Chhavi1 aShrivastava, Sadhana1 aSrivastava, Nalini1 aNarayana, Sunil, Kumar Kopp1 aShukla, Sangeeta uhttps://www.phcogres.com/article/2017/9/2/1041030974-8490204643