02646nas a2200229 4500008004100000245012500041210006900166260001400235300001200249490000700261520188500268653002602153653002102179653002102200653003102221100001602252700002002268700002102288700002202309700002302331856006202354 2018 eng d00aProtective Effect of Combretum Hypopilinum Diels: Root Bark Extract against CCl4‑Induced Hepatotoxicity in Wistar Rats0 aProtective Effect of Combretum Hypopilinum Diels Root Bark Extra cJuly 2018 a325-3310 v103 a

Background: Combretum hypopilinum is used in Togolese traditional medicine to alleviate various ailments including hepatic disorders. Objective: This study evaluates the hepatoprotective activities of crude ethanolic extract of C. hypopilinum root bark (CECH) and its possible mechanism(s) of action in Wistar rats. Materials and methods: In vitro antioxidant potential was evaluated using DPPH, Ferric-reducing power and hydroxyl radical scavenging assays. Hepatoprotective activity was assessed using CCl4-induced acute hepatotoxicity in rats. Animals were pretreated orally with different doses of CECH (200 and 400 mg/kg) once daily for 7 consecutive days and were subjected to intraperitoneal single injection of CCl4 (1 mL/kg). Serum markers of hepatic damage, hepatic endogenous antioxidants, NO, TNF-α and lipid peroxidation contents were measured out. Results: The pretreatment with CECH significantly decreased levels of hepatic enzymes (p < 0.05, p < 0.01, p < 0.001) and MDA in pretreated rats (p < 0.05, p < 0.01). CECH restored the decreased levels of hepatic antioxidants including SOD (p < 0.01), CAT (p < 0.05 at 400 mg/kg) and GSH (p < 0.05 at 400 mg/kg) and significantly inhibited hepatic production of NO (p < 0.05, p < 0.01). as well as the level of TNF-α (p < 0.001) in pretreated rats. Protective effects of CECH were confirmed by histopathological examinations. CECH exhibited antioxidant in vitro and contains also total phenol and flavonoid. Conclusion: This study suggests that CECH protected the liver from CCl4-induced hepatic damage possibly via antioxidant and anti-inflammatory mechanisms.

10aCombretum hypopilinum10aHepatoprotective10aOxidative stress10aTumor necrosis factor‑α1 aIdoh, Kokou1 aDosseh, Kossivi1 aKpatcha, Tchazou1 aAgbonon, Amegnona1 aGbeassor, Messanvi uhttps://www.phcogres.com/article/2018/10/3/104103prpr2717