03054nas a2200301 4500008004100000245012300041210006900164260001800233300001100251490000600262520204700268653002402315653001702339653002402356653001502380653001402395653001002409100002902419700002702448700002802475700003202503700002602535700003202561700003202593700002802625700003102653856006802684 2017 eng d00aAntifungal Activity of Hydroalcoholic Extract of Chrysobalanus icaco Against Oral Clinical Isolates of Candida Species0 aAntifungal Activity of Hydroalcoholic Extract of Chrysobalanus i cFebruary 2017 a96-1000 v93 a

Background: Chrysobalanus icaco is a medicinal plant commonly used to treat fungal infections in Brazilian Amazonian region. Objective: This work aimed to evaluate the antifungal activity of the hydroalcoholic extract of C. icaco (HECi) against oral clinical isolates of Candida spp. and to determine the pharmacognostic parameters of the herbal drug and the phytochemical characteristics of HECi. Materials and Methods: The pharmacognostic characterization was performed using pharmacopoeial techniques. Phytochemical screening, total flavonoid content, and high-performance liquid chromatography (HPLC) analysis were used to investigate the chemical composition of the HECi. A broth microdilution method was used to determine the antifungal activity of the extract against 11 oral clinical isolates of Candida spp. Results: Herbal drug presented parameters which were within the limits set forth in current Brazilian legislation. A high amount of flavonoid content (132,959.33 ± 12,598.23 μg quercetin equivalent/g of extract) was found in HECi. Flavonoids such as myricetin and rutin were detected in the extract by HPLC analyses. HECi showed antifungal activity against oral isolates of Candida albicans and Candida parapsilosis (minimum inhibitory concentrations [MIC] 3.12 and 6.25 mg/mL, respectively), and C. albicans American American Type Culture Collection (MIC <1.56 mg/mL). Conclusion: HECi was shown to possess antifungal activity against Candida species with clinical importance in the development of oral candidiasis, and these activities may be related to its chemical composition. The antifungal activity detected for C. icaco against Candida species with clinical importance in the development of oral candidiasis can be attributed to the presence of flavonoids in HECi, characterized by chromatographic and spectroscopic techniques.

10aAntifungal activity10aCandida spp.10aChrysobalanus icaco10aFlavonoids10aMyricetin10aRutin1 aSilva, João, Paulo Bast1 aPeres, Ana, Regina Mau1 aPaixão, Thiago, Portal1 aSilva, Andressa, Santa Brí1 aBaetas, Ana, Cristina1 aBarbosa, Wagner, Luiz Ramos1 aBarbosa, Wagner, Luiz Ramos1 aMonteiro, Marta, Chagas1 aAndrade, Marcieni, Ataíde uhttps://www.phcogres.com/article/2017/9/1/1041030974-8490199772