02887nas a2200265 4500008004100000245013900041210006900180260001500249300001200264490000700276520196700283653002502250653001102275653001702286653002102303653001802324100003002342700002902372700003402401700003102435700003202466700002902498700003102527856006302558 2018 eng d00aPhytochemical Screening, Total Phenolic Content, Antioxidant, and Cytotoxic Activity of Five Peruvian Plants on Human Tumor Cell Lines0 aPhytochemical Screening Total Phenolic Content Antioxidant and C cApril 2018 a161-1650 v103 a

Background: Cancer is considered a serious public health problem in the world; searching alternative treatments of medicinal plants constitute a promissory field to find new anticancer drugs. Objective: The aim of this study is to evaluate the phytochemical screening, total phenolic content (TPC), antioxidant, and cytotoxic activity of ethanol extracts of Waltheria ovata (WO), Piper aduncum (PA), Myrciaria dubia (MD), Physalis peruviana (PP), and Geranium dielsianum (GD) on human tumor cell lines. Materials and Methods: Phytochemical screening was assessed using chemical reactives. TPC was developed using Folin Ciocalteu reactive, and the antioxidant activity was determined against 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH), and 2,2‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulfonic acid) (ABTS) radicals by spectrophotometry. The cytotoxic activity was determined on human tumor cell lines followed as: MCF‑7, H‑460, HT‑29, M‑14, K‑562, and DU‑145. Results: Phytochemical study confirmed flavonoids and phenolic compounds in all extracts. TPC was found to be the highest in WO extract (1250 mg of gallic acid equivalent/g of dried extract) rather than other extracts. The highest antioxidant activity was stablished in WO extract for DPPH and ABTS radical scavenging tests (inhibitory concentration [IC50] = 0.89 ± 0.01 μg/mL, IC50 = 4.20 ± 1.50 μg/mL). Ethanolic extracts (μg/mL) showed low cytotoxicity on human tumor cell lines (CI50 >20 μg/mL) for PA, PP, GD, MD. Meanwhile, WO presented cytotoxicity on H460, MCF‑7, and K562 tumor cell lines. Conclusion: In our findings, WO and MD extracts revealed a high antioxidant and TPC. WO exhibited better cytotoxic effect compared with 5‑FU. Hence, these medicinal plants could be effective to prevent cancer disease and oxidative stress.

10aAntioxidant activity10aCancer10acytotoxicity10aOxidative stress10aPhytochemical1 aHerrera‑Calderon, Oscar1 aAlvarado‑Puray, Corina1 aArroyo‑Acevedo, Jorge, Luis1 aRojas‑Armas, Juan, Pedro1 aChumpitaz‑Cerrate, Victor1 aHañari‑Quispe, Renán1 aValenzuela‑Herrera, Rita uhttps://www.phcogres.com/article/2018/10/2/104103prpr10917