02740nas a2200265 4500008004100000245019000041210006900231260001800300300001000318490000600328520183000334653000902164653003002173653004002203653002902243653001702272653000902289653001002298653002702308653000702335100002302342700002202365700001902387856006802406 2017 eng d00aCytotoxicity Studies of the Extracts, Fractions, and Isolated Compound of Pseudocedrela kotschyi on Cervical Cancer (HeLa), Breast Cancer (MCF‑7) and Skeletal Muscle Cancer (RD) Cells0 aCytotoxicity Studies of the Extracts Fractions and Isolated Comp cFebruary 2017 a46-500 v93 a

Background: This study determined the cytotoxic effects of root and stem bark extracts, fractions, and isolated compounds derived from Pseudocedrela kotschyi on HeLa, MCF-7, and RD cells. Materials and Methods: The cytotoxic activity was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay against three cell lines (RD, HeLa, and MCF 7) at concentrations ranging from 0.01 to 1000 μg/mL. Isolation of crude saponin was done from the most active ethyl acetate fraction and further purified using vacuum liquid chromatography and preparative thin layer chromatographic techniques. Results: The cytotoxicity assay revealed that the methanol extract from the root bark and the ethyl acetate fraction from the stem bark exhibited marked anticancer activity with IC50 of 87.36 μg/ml and 21.53 μg/ml, respectively, on HeLa cancer cell line and 101.51 μg/mL and 38.46 μg/mL, respectively, on RD cell line. These values are comparable with that obtained from vinblastine and methotrexate used as standard drugs (IC50 values of 0.01 μg/mL and 0.05 μg/mL, respectively). The isolated crude saponins also gave IC50 values of 5.28 μg/mL and 81.52 μg/mL against the RD cell lines and IC50 values of 1.05 μg/mL and 86.8 μg/mL for the MCF 7 cancer cell lines. PTLC led to the isolation of a compound from the crude saponin which was identified as 7-deacetoxy-7-oxogedunin through spectroscopic analysis and comparison with literature data. Conclusions: P. kotschyi could be considered as a potential source of chemotherapeutic agent. However, further research to determine the exact mechanism of action needs to be carried out.

10a3-(410a5-dimethylthiazol-2-yl)-210a5-diphenyltetrazolium bromide assay10a7-deacetoxy-7-oxogedunin10acytotoxicity10aHeLa10aMCF-710aPseudocedrela kotschyi10aRD1 aElufioye, Taiwo, O1 aAbdul, Abolaji, A1 aMoody, Jone, O uhttps://www.phcogres.com/article/2017/9/1/1041030974-8490199776