02782nas a2200217 4500008004100000245018600041210007100227260001800298300000900316490000700325520196700332653002302299653003602322653002502358653002502383653002102408653003502429100002002464700001702484856006302501 2019 eng d00aSaponin of Momordica cymbalaria Exhibits Anti‑Inflammatory Activity by Suppressing the Expression of Inflammatory Mediators in Lipopolysaccharide‑Stimulated RAW264.7 Macrophages0 aSaponin of Momordica cymbalaria Exhibits Anti‑Inflammatory Activ cFebruary,2019 a8-130 v113 a

Background: Inflammation is an intricate biological process that commonly occurs in response to pathologic stimuli, and natural products have potential in healing inflammation. However, the anti‑inflammatory of Momordica cymbalaria is not evaluated yet. Objectives: The anti‑inflammatory mechanism of saponin of M. cymbalaria (SMC) was investigated in bacterial lipopolysaccharide (LPS)‑stimulated RAW264.7 mouse macrophage cell line. Materials and Methods: The cytotoxicity of SMC on RAW264.7 cells was determined by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay at 500, 250, 125, 62.5, 31.25, 15.625, 7.812, 3.906, and 1.953 μg/mL. For anti‑inflammatory activity, RAW264.7 cells were stimulated with Escherichia coli LPS (1 μg/ml) in the presence or absence of SMC (50 μg/ml) for 16–24 h. Western blotting was carried to comprehend the expression of cyclooxygenase‑2 (COX‑2) and inducible nitric oxide (NO) synthase (iNOS) whereas expressions of the pro‑inflammatory cytokines (interleukin [IL]‑6, IL‑1β, and tumor necrosis factor‑alpha [TNF‑α]) and prostaglandin E2 (PGE2) were studied by enzyme‑linked immunosorbent assay (ELISA). NO production was estimated by Griess’s method. Salicylic acid, a nonsteroidal anti‑inflammatory drug, was used as a standard. Results: The saponin did not exert significant cytotoxicity on RAW264.7 cells. Western blot analysis revealed reduction in COX‑2 and iNOS expression on SMC treatment. Production of PGE2, IL‑6, IL‑1β, and TNF‑α was also found to be reduced when analyzed by ELISA. NO levels were also lowered. Conclusion: The findings suggest that the SMC possesses potential anti‑inflammatory activity by suppressing the expression of inflammatory mediators, COX‑2, iNOS, PGE2, and NO, and the cytokines in LPS‑stimulated RAW264.7 cells.

10aCyclooxygenase‑210aInducible nitric oxide synthase10aInterleukin‑1 beta10aMomordica cymbalaria10aProstaglandin E210aTumor necrosis factor‑alpha.1 aSamaddar, Suman1 aKoneri, Raju uhttps://www.phcogres.com/article/2019/11/1/104103prpr13018