02360nas a2200289 4500008004100000245019200041210006900233260001800302300001000320490000600330520132200336653003401658653003101692653006201723653002001785653003601805100001801841700001601859700001601875700001901891700001701910700002101927700001601948700001801964700002001982856006802002 2017 eng d00aAnalysis of Soluble Proteins in Natural Cordyceps sinensis from Different Producing Areas by Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis and Two-dimensional Electrophoresis0 aAnalysis of Soluble Proteins in Natural Cordyceps sinensis from  cFebruary 2017 a34-380 v93 a

Background: As one of the bioactive components in Cordyceps sinensis (CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS. Objective: Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas. Materials and Methods: Proteins from 26 different producing areas were extracted by Tris-HCl buffer with Triton X-100, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). Results: The SDS-PAGE results indicated that the number of protein bands and optical density curves of proteins in 26 CS samples was a bit different. However, the 2-DE results showed that the numbers and abundance of protein spots in protein profiles of 26 samples were obviously different and showed certain association with producing areas. Conclusions: Based on the expression values of matched protein spots, 26 batches of CS samples can be divided into two main categories (Tibet and Qinghai) by hierarchical cluster analysis.

10aHierarchical cluster analysis10aNatural Cordyceps sinensis10aSodium dodecyl sulfate-polyacrylamide gel electrophoresis10aSoluble protein10aTwo-dimensional electrophoresis1 aLi, Chun-Hong1 aZuo, Hua-Li1 aZhang, Qian1 aWang, Feng-Qin1 aHu, Yuan-Jia1 aQian, Zheng-Ming1 aLi, Wen-Jia1 aXia, Zhi-Ning1 aYang, Feng-Qing uhttps://www.phcogres.com/article/2017/9/1/1041030974-8490199782