03139nas a2200301 4500008004100000245011700041210006900158260001800227300000800245490000600253520215600259653002802415653001502443653001002458653002902468653001902497100002302516700002202539700003602561700002302597700002202620700002302642700002302665700003202688700002302720700002602743856006802769 2017 eng d00aAntihyperglycemic Activity of the Leaves from Annona cherimola Miller and Rutin on Alloxan-induced Diabetic Rats0 aAntihyperglycemic Activity of the Leaves from Annona cherimola M cFebruary 2017 a1-60 v93 a

Background: Annona cherimola, known as “chirimoya” has been reported in Mexican traditional medicine for the treatment of diabetes. Objective: The aims of the present study were to validate and assess the traditional use of A. cherimola as an antidiabetic agent. Materials and Methods: The ethanol extract from A. cherimola (300 mg/ kg, EEAc), subsequent fractions (100 mg/kg), and rutin (30 mg/kg) were studied on alloxan-induced type 2 diabetic (AITD) and normoglycemic rats. In addition, oral glucose tolerance test (OGTT) and oral sucrose tolerance test (OSTT) were performed in normoglycemic rats. Molecular docking technique was used to conduct the computational study. Results: Bioassay-guided fractionation of EEAc afforded as major antihyperglycemic compound, rutin. EEAc attenuated postprandial hyperglycemia in acute test using AITD rats (331.5 mg/dL) carrying the glycemic levels to 149.2 mg/ dL. Rutin after 2 h, attenuated postprandial hyperglycemia in an acute assay using AITD rats such as EEAc, with maximum effect (150.0 mg/dL) being seen at 4 h. The antihyperglycemic activities of EEAc and rutin were comparable with acarbose (151.3 mg/dL). In the subchronic assay on AITD rats, the EEAc and rutin showed a reduction of the blood glucose levels since the 1st week of treatment, reaching levels similar to normoglycemic state (116.9 mg/kg) that stayed constant for the rest of the assay. OGTT and OSTT showed that EEAc and rutin significantly lowered blood glucose levels in normoglycemic rats at 2 h after a glucose or sucrose load such as acarbose. Computational molecular docking showed that rutin interacted with four amino acids residues in the enzyme α-glucosidase. Conclusion: The results suggest that rutin an α-glucosidase inhibitor was responsible in part of the antihyperglycemic activity of A. cherimola. Its in vivo antihyperglycemic activity is in good agreement with the traditional use of A. cherimola for the treatment of diabetes.

10aAnnona cherimola Miller10aAnnonaceae10aRutin10aType 2 diabetes mellitus10aα-Glucosidase1 aCalzadax, Fernando1 aCalzada, Fernando1 aSolares-Pascasio, Jesús, Iván1 aOrdoñez-Razox, RM1 aOrdoñez-Razo, RM1 aVelazquez, Claudia1 aBarbosa, Elizabeth1 aGarcía-Hernández, Normand1 aMendez-Luna, David1 aCorrea-Basurto, José uhttps://www.phcogres.com/article/2017/9/1/1041030974-8490199781