02611nas a2200229 4500008004100000245009800041210006900139260001800208300001200226490000700238520190400245653001502149653001102164653002802175653001102203100001602214700001802230700002402248700002502272700002102297856006302318 2018 eng d00aAssessment of In vivo Antiviral Potential of Datura metel Linn. Extracts against Rabies Virus0 aAssessment of In vivo Antiviral Potential of Datura metel Linn E cFebruary 2018 a109-1120 v103 a

Objective: The soxhlet, cold, and ayurvedic extracts of Datura metel Linn. were evaluated for in vivo antirabies activity. Materials and Methods: Soxhlet and cold extraction method were used to extract Datura (fruit and seed) extracts, and ayurvedic extraction of Datura was prepared. In vivo toxicity assay was performed as per the OECD 420. LD50 dose was calculated by Reed and Muench method. The in vivo antirabies activity was screened in Swiss albino mice with the virus challenge dose of 10 LD50 (intracerebrally) in both preexposure (PE) and postexposure treatment with oral administration of Datura extracts in Swiss albino mice and observed for 21 days. The virus load in the mice brain was evaluated by TCID50 titration method. Results: Datura (ayurvedic preparation) was found to be nontoxic up to 2000 mg/kg in Swiss albino mice, i.e., 60 mg/30 g of mice, when administered (0.5 ml) orally and observed till 21 days. Up to 20% survival rate on the test group (PE of Datura extracts) up to 14 days postinfection as compared to the virus control group (10 LD50) was observed. No survival rate was observed in the postexposure group of Datura extract; however, the survival time was increased by 4 days as compared to the virus control group. Viral load of the infected mice brain sample was estimated in vero cell line, and 3 log reduction in the virus titer was observed in text group as compared to the virus control, suggesting that Datura extract has an in vivo antirabies activity. Conclusion: To the best of our knowledge, this is the first study of in vivo antiviral activity of an ayurvedic preparation of D. metel Linn. against rabies virus. Datura extracts have a potential in vivo antirabies activity.

10aAntirabies10aDatura10aIn vivo antiviral assay10aRabies1 aRoy, Soumen1 aSamant, Lalit1 aGanjhu, Rajeshkumar1 aMukherjee, Sandeepan1 aChowdhary, Abhay uhttps://www.phcogres.com/article/2018/10/1/104103prpr12117