03347nas a2200349 4500008004100000245005600041210005200097260001800149300000900167490000700176520234300183653001602526653001802542653001702560653004202577653001402619653000902633653001202642653001202654653001902666653003802685653001302723653001702736653003202753653001802785653002402803100003102827700002102858700002802879700002802907856006202935 2018 eng d00aCytotoxic Compounds from Wrightia pubescens (R.Br.)0 aCytotoxic Compounds from Wrightia pubescens RBr cFebruary 2018 a9-150 v103 a

Background: Mixtures of ursolic acid (1) and oleanolic acid (2) (1:1 and 1:2), oleanolic acid (2), squalene (3), chlorophyll a (4), wrightiadione (5), and α‑amyrin acetate (6) were isolated from the dichloromethane (CH2Cl2) extracts of the leaves and twigs of Wrightia pubescens (R.Br.). Objectives: To test for the cytotoxicity potentials of 1–6. Materials and Methods: The antiproliferative activities of 1–6 against three human cancer cell lines, breast (MCF‑7) and colon (HT‑29 and HCT‑116), and a normal cell line, human dermal fibroblast neonatal (HDFn), were evaluated using the PrestoBlue® cell viability assay. Results: Compounds 4, 1 and 2 (1:2), 2, 1 and 2 (1:1), and 5 exhibited the most cytotoxic effects against HT‑29 with half maximal inhibitory concentration (IC50) values of 0.68, 0.74, 0.89, 1.70, and 4.07 μg/mL, respectively. Comparing 2 with its 1:1 mixture with 1 (IC50 = 1.70 and 7.18 μg/mL for HT‑29 and HCT‑116, respectively) and 1:2 mixture with 1 (0.74 and 3.46 μg/mL for HT‑29 and HCT‑116, respectively), 2 also showed strong cytotoxic potential against HT‑29 and HCT‑116 (0.89 and 2.33 μg/mL, respectively). Unlike the mixtures which exhibited low effects on MCF‑7 (IC50 = 20.75 and 30.06 μg/mL for 1:1 and 1:2, respectively), 2 showed moderate activity against MCF‑7 (10.99 μg/mL). Compound 6 showed the highest cytotoxicity against HCT‑116 (IC50 = 4.07 μg/mL). Conclusion: Mixtures of 1 and 2 (1:1 and 1:2), 2, 3, 4, 5, and 6 from the CH2Cl2 extracts of the leaves and twigs of W. pubescens (R.Br.) exhibited varying cytotoxic activities. All the compounds except 6 exhibited the strongest cytotoxic effects against HT‑29. On the other hand, 6 was most cytotoxic against HCT‑116. Overall, the toxicities of 1–6 were highest against HT‑29, followed by HCT‑116 and MCF‑7. All the compounds showed varying activities against HDFn (IC50 <30 μg/mL).

10aApocynaceae10aChlorophyll a10acytotoxicity10aHalf maximal inhibitory concentration10aHCT‑11610aHDFn10aHT‑2910aMCF‑710aOleanolic acid10aPrestoBlue® cell viability assay10aSqualene10aUrsolic acid10aWrightia pubescens R. Brown10aWrightiadione10aα‑amyrin acetate1 aReyes, Mariquit, M. De Los1 aOyong, Glenn, G.1 aNg, Vincent, Antonio S.1 aRagasa, Consolacion, Y. uhttps://www.phcogres.com/article/2018/10/1/104103prpr4517