02528nas a2200265 4500008004100000245011200041210007100153260001600224300001200240490000700252520167100259653001901930653002201949653002101971653001601992653002002008100002702028700002802055700001602083700001902099700002902118700001702147700003602164856006202200 2020 eng d00aCitrus limon L. Gel Stimulate Lymphocytes and Interleukin‑10 Expression in Traumatic Diabetic Oral Ulcers0 aCitrus limon L Gel Stimulate Lymphocytes and Interleukin‑10 Expr cAugust 2020 a299-3020 v123 a

Background: Citrus limon L. and its peel report anti‑inflammatory, antioxidant, anti-fungal, and anti-bacterial properties. However, the effects of C. limon L. gel to treat traumatic oral ulcers on diabetic conditions have yet to be investigated. Purpose: This study aims to discover the potent effect of C. limon L. on traumatic diabetic oral ulcers, in terms of both lymphocytes and interleukin (IL)‑10 expression. Materials and Methods: C. limon L. gel was made from C. limon L. peel and was extracted by means of steam distillation. Twenty male, diabetic Wistar rats with traumatic ulcers on the inferior labial mucosa were divided into four groups (n = 5). The control groups were treated with 3% carboxylmethyl celullosa natrium and the treatment groups were treated with C. limon L. gel. Each treatment was administered once a day for either 5 or 7 days. Lymphocytes were analyzed by means of hematoxylin and eosin staining and IL‑10 expression by immunohistochemistry staining. An independent t‑test (P < 0.05) was used to analyze and determine the number of lymphocytes and IL‑10 expression. Results: The number of lymphocytes and IL‑10 expression in the treatment groups on the fifth and 7th days was higher than those in the control groups (P < 0.05). Conclusion: The essential oil in C. limon L. peel can be used to treat traumatic diabetic oral ulcers, as it is able to stimulate the number of lymphocytes and IL‑10 expression.

10aCitrus limon L10aDiabetes mellitus10aInterleukin‑1010aLymphocytes10aTraumatic ulcer1 aMahdani, Fatma, Yasmin1 aErnawati, Diah, Savitri1 aHadi, Priyo1 aSoebadi, Bagus1 aMardiyana, Shiffin, Devi1 aSusanti, Dwi1 aSurboyo, Meircurius, Dwi Condro uhttps://www.phcogres.com/article/2020/12/3/104103prpr1220