02162nas a2200217 4500008004100000245011200041210006900153260001600222300001400238490000700252520147500259653001901734653001601753653001701769653001301786653001401799653002001813100002301833700002201856856006601878 2025 eng d00aComparative HPTLC Analysis of Piperine Content in Roots of Piper longum L. Cultivated with Bio Formulations0 aComparative HPTLC Analysis of Piperine Content in Roots of Piper cAugust 2025 a1308-13140 v173 a

Background: Phytochemicals from medicinal plants significantly impact healthcare. Piperine, a bioactive compound in Piper longum, exhibits diverse pharmacological effects, including analgesic, antipyretic, antioxidant, immune-stimulant, hepatoprotective, and bioavailability-enhancing properties. Traditionally, Piperine is extracted from both fruits (Pippali) and roots (Pippalimula) of Piper longum. Objectives: To quantify the Piperine content in Piper longum roots cultivated using bio formulations-Kunapa Jala and Vermiwash-on Piperine yield. Materials and Methods: Piper longum was cultivated with Random Block Design with two treatment groups (Kunapa Jala and Vermiwash), each with 12 replications. Roots were harvested, cleaned, dried, and powdered. High-Performance Thin-Layer Chromatography (HPTLC) was used to detect and quantify of Piperine. Results: HPTLC analysis revealed the presence of Piperine in both groups. Quantitative comparison showed a higher Piperine concentration in the Kunapa Jala treated group than in the Vermiwash group. Conclusion: Kunapa Jala enhances Piperine content in Piper longum roots in comparison to Vermiwash. This supports the potential of Vrukshayurveda and traditional formulations in improving the phytochemical yield of Pippali.

10aHPTLC analysis10aKunapa Jala10aPiper longum10aPiperine10aVermiwash10aVrukshaayurveda1 aHoleyache, Adavesh1 aChaudhary, Vishal uhttps://www.phcogres.com/article/2025/17/4/105530pres20252328