02343nas a2200217 4500008004100000245014600041210006900187260001600256300000800272490000700280520161100287653002201898653001601920653001001936653001401946653002601960100002001986700002602006700002502032856006802057 2025 eng d00aInvestigating the Expression of Apoptosis and Metastasis Genes in Satureja khuzistanica Along with Nisin and Doxorubicin in Colorectal Cancer0 aInvestigating the Expression of Apoptosis and Metastasis Genes i cAugust 2025 a1-80 v173 a

Background: Colorectal cancer is the third most common cancer and the second most common cause of cancer-related death worldwide. Common treatments for colorectal cancer include chemotherapy, surgery, immunotherapy, etc. One of the chemotherapy drugs is doxorubicin, but it has many side effects. Recently, the antibacterial and anticancer effects of Satureja khuzistanica (S. khuzistanica) extract have been proven. Probiotics have been used in recent years to change the intestinal microbiome and treat colon cancer and reduce the side effects of treatments. Material and Methods: In this study, the viability of HT29 colorectal cancer cells was examined using MTT after treatment with the study groups, and the expression of BAX, BCL2, SMAC, P53, MMP9, CASP9, and SUR genes was evaluated by Real time PCR. Results: The results showed that the expression of BAX, SMAC, P53, and CASP9 genes increased by 4.21, 3.055, 5.105, and 2.216 (p<0.0001) compared to the control after treatment with S. khuzistanica, respectively. The expression of BCL2, MMP2, SUR and MMP9 genes decreased by 1.1% after treatment with S. khuzistanica compared to the control. The results also showed that the combined treatment of S. khuzistanica with Nisin or doxorubicin had a greater effect on changes in gene expression. Conclusion: This study could be a therapeutic strategy for using natural substances such as S. khuzistanica extract and probiotic Nisin to reduce the dose of chemotherapy.

10aColorectal Cancer10aDoxorubicin10aNisin10aProbiotic10aSatureja khuzistanica1 aKheiri, Esmaeil1 abonab, Zahra, Hojjati1 aSoltanzadeh, Hossein uhttps://www.phcogres.com/article/2025/17/4/105530pres20252194-0