02527nas a2200253 4500008004100000245011100041210006900152260001400221300001400235490000700249520171600256653001701972653001201989653001902001653001302020653003002033653001902063100002702082700003402109700001602143700001802159700003002177856006602207 2025 eng d00aPhytochemical Extraction, Screening, GCMS Analysis and Antioxidant Properties of Ophiorrhiza recurvipetala0 aPhytochemical Extraction Screening GCMS Analysis and Antioxidant cJune 2025 a1050-10610 v173 a

Background and Objectives: Most of the people from developing countries depend on plant-based ailments for several diseases due to the occurrence of pharmacological bioactive substances with lowered adverse effects. Ophiorrhiza recurvipetala (OR), a newly identified species from the Rubiaceae family, which was unexplored for its bioactive compounds and antioxidant properties. Materials and Methods: The whole plant samples were collected, authenticated extracted with ethanol and n-hexane, subjected to phytochemical analysis and analyzed by GC-MS. Antioxidant activity of the extracts were assessed using FRAP, DPPH, ABTS, hydroxyl, and superoxide radical scavenging assays. Results: The phytochemical examination explored the presence of several phytochemicals including flavonoids, alkaloids, phenols, steroids, tannins, glycosides, and saponins in the ethanolic (OREE) and n-Hexane (ORNHEX) extracts. GC-MS analysis identified 58 and 81 compounds in OREE and ORNHEX, respectively. Although the results demonstrated that both extracts exhibited significant antioxidant properties, OREE displayed superior radical scavenging activity which was evidenced by IC50 values. Conclusion: These findings suggest that O. recurvipetala has potential bioactive compounds and could serve as a valuable source of natural antioxidants for therapeutic use. This study provides a foundation for further research aimed in isolating and identifying novel compounds with potential applications in medicine and drug development. As far as we understand, this is the first study on this plant species.

10aAntioxidants10aethanol10aGC-MS Analysis10an-Hexane10aOphiorrhiza recurvipetala10aPhytochemicals1 aVidya, Venkatakrishnan1 aThenmozhi, Arokiasamy, Justin1 aSurya, Ravi1 aPrema, Asokan1 aManivasagam, Thamilarasan uhttps://www.phcogres.com/article/2025/17/3/105530pres20252230