02166nas a2200217 4500008004100000245012200041210006900163260001400232300001200246490000700258520147000265653001301735653001701748653001701765653001601782653001501798100002101813700002701834700002101861856006601882 2025 eng d00aFabrication of Snail Mucin-Silk Fibroin Hydrogel Scaffold for Guided Tissue Regeneration: A Zebrafish Fin Model Study0 aFabrication of Snail MucinSilk Fibroin Hydrogel Scaffold for Gui cJune 2025 a927-9330 v173 a

Background: Scaffolds play a pivotal role in guiding tissue regeneration by providing a suitable environment for cell growth, migration, and differentiation. Among various materials, biopolymers such as silk fibroin and snail mucin have garnered attention due to their biocompatibility, natural origin, and potential to promote cellular activities. Objectives: In this study, we explore the fabrication of a novel Snail Mucin-Silk Fibroin hydrogel scaffold designed to enhance tissue regeneration. Materials and Methods: The hydrogel scaffold has been prepared by mixing 28% w/v gelatin, 21% w/v PVA in distilled water and 10% HPMC, and heated to 40ºC followed by addition of a snail mucin and silk fibroin at a lower temperature and freeze dried at 4ºC for 12 hr. Results: The characterization tests showed the scaffold’s favourable biological properties and morphology, such as biocompatibility, hemocompatibility, ability to retain moisture, and promotion of cellular activities essential for tissue repair. Histopathological analysis showed that the snail mucin-silk fibroin hydrogel scaffold significantly enhanced fin regeneration compared to the control groups. Conclusion: Thus, the snail mucin hydrogel used in this study showed better soft tissue regeneration potential and hence be used for various applications in regenerative medicine.

10aHydrogel10aRegeneration10aSilk Fibroin10aSnail Mucin10aZebra Fish1 aSebastian, Sofia1 aPurushothaman, Bargavi1 aRamani, Pratibha uhttps://www.phcogres.com/article/2025/17/3/105530pres20252176