02586nas a2200229 4500008004100000245011400041210006900155260001500224300001200239490000700251520184400258653001402102653001402116653002802130653002502158653001802183100002202201700002202223700002502245700002002270856006602290 2025 eng d00aPhyto-Analytical Studies and in vitro Wound Healing Activity of Synedrella nodiflora (L.) Gaertn Leaf Extract0 aPhytoAnalytical Studies and in vitro Wound Healing Activity of S cApril 2025 a519-5310 v173 a

Background: Traditionally among the Nigerian population Synedrella nodiflora is prominently used for treating Putrefied Wound. Objectives: The present work was designed to evaluate the In vitro Wound healing activity of Synedrella nodiflora leaf extracts. Materials and Methods: The current research work was aimed to perform the Qualitative phytochemical screening, FTIR, ICP-OES, Quantitative estimation of total phenolic and flavonoid content and in vitro pharmacological assay includes Antioxidant assay, Protein denaturation assay, cell line scratch assay for wound healing of EAE and EEs of Synedrella nodiflora. Results: Secondary phytochemicals like Flavonoid, Alkaloid, Tannin, Phenols, Terpenoids and Steroids were reported. The ICP-OES analysis concluded that the presence of elements like Zn, Fe, Cu, Mg and heavy metals like Ni, Pb, Cr and Cd were within their limits as per WHO. The EAE and EE of Synedrella nodiflora were assessed for their total phenolic and flavonoid content. The Antioxidant activity of EAE and EE showed IC50 value 2.24±0.02 mg/mL and 3.72±0.02 mg/mL compared with standard Ascorbic Acid of 42.76±0.01 μg/mL. The % Inhibition of standard Diclofenac shows IC50 value 27.99±0.026 μg/mL. whereas the IC50 values of EAE and EE were found to be 294.98±0.02 μg/mL and 138.98±0.05 μg/mL respectively. The relative cell migration of EAE and EE were 0.041±0.05 mm and 0.036±0.01 mm at 2mcg. Conclusion: These studies concluded that the Synedrella nodiflora leaf extracts were rich in various phytochemicals and can be used for treating wounds due to its antioxidant, anti-inflammatory and wound healing potential.

10aFlavonoid10aPhenolics10aPhytochemical screening10aSynedrella nodiflora10aWound healing1 aKoodathil, Joshna1 aRamadass, Lathika1 aJayachandran, Sindhu1 aSekar, Jaganath uhttps://www.phcogres.com/article/2025/17/2/105530pres20251916