02973nas a2200277 4500008004100000245006900041210006800110260001700178300001000195490000700205520217400212653002302386653001202409653001302421653000902434653001202443653000802455653000802463100002802471700003302499700002702532700002302559700002102582700002602603856006602629 2025 eng d00aAnalytical Characterization of Achyranthes aspera L. Whole Plant0 aAnalytical Characterization of Achyranthes aspera L Whole Plant cJanuary 2025 a85-930 v173 a

Background: The therapeutic properties of Achyranthes aspera have long been acknowledged and integrated into diverse traditional healthcare like Ayurvedic, Siddha, Unani and Naturopathic medicine for the management of various ailments. In ancient Indian systems of medicine such as Siddha and Ayurveda, the constituents found in this medicinal plant play a pivotal role in treating disorders. Both macro and micro elements are crucial components for plants to carry out their basic functions. Objectives: Consequently, the ash of Achyranthes aspera was analyzed chemically, with a focus on phytochemical and chromatographic analysis, along with spectral characterization of the entire plant, to determine the presence of inorganic constituents. Materials and Methods: Standard methods were followed for all physicochemical parameters. Chromatographic characterization was carried out using High Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC). Additionally, elemental characterization was performed using different instruments such as Energy Dispersive X-ray analysis (EDX), X-ray Fluorescence (XRF), Powder X-ray Diffraction (PXRD), X-ray Photoelectron Spectroscopy (XPS) and Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) to investigate the minerals present in the ash of Achyranthes aspera. Results: The HPTLC photo documentation revealed the presence of five spots at 254 nm and eight spots at 366 nm. Vanillin sulfuric acid reagent derivatization resulted in the appearance of 9 spots under white light. Furthermore, the HPLC chromatogram showed the separation of 13 peaks at 366 nm. The evaluation techniques also detected various elements such as Carbon (C), Oxygen (O), Sodium (Na), Magnesium (Mg), Aluminium (Al), Silicon (Si), Phosphorus (O), sulphur (S), Chlorine (Cl), potassium (K), Calcium (Ca), Manganese (Mn), Chromium (Cr), Iron (Fe) and Zinc (Zn). Conclusion: The analysis primarily focuses on quality control criteria and the mineral composition of the plant.

10aAchyranthes aspera10aICP-OES10aNayurivi10aPXRD10aSEM-EDX10aXPS10aXRF1 aMandal, Achintya, Kumar1 aMeenakshisundaram, Saravanan1 aSubramanian, Subashini1 aThatipelli, Sujith1 aChandran, Rajesh1 aRamachandran, Shakila uhttps://www.phcogres.com/article/2025/17/1/105530pres20251928