02378nas a2200241 4500008004100000245009300041210006900134260001700203300001000220490000700230520162200237653001701859653001801876653001401894653001901908653001801927100002601945700002401971700002201995700002702017700002602044856006602070 2025 eng d00aStandardization of Karisalai Madakku Tailam and Quantification of Wedelolactone by HPTLC0 aStandardization of Karisalai Madakku Tailam and Quantification o cJanuary 2025 a78-840 v173 a

Background: Herbal oils also known as Medicated oils, are oils infused with medicinal properties of herbs herbs plants. Objectives: To prepare the herbal oil, Karisalai tailam using gingelly oil and Eclipta alba Linn. (Karisalai) further to continue the process of madakku for two more time and to investigate the physico-chemical parameters for any changes. Materials and Methods: The herbal oil was prepared as per literature. Acid, saponification, iodine, peroxide values, rancidity, refractive index were estimated for the raw gingelly oil, gingelly oil heated in parallel without adding Eclipta alba Linn. juice, Karisalai Madakku Tailam (KMT1), second Madakku Tailam (KMT2) and third madakku tailam (KMT3). The content of major coumestane derivative present in Eclipta alba Linn., ie., wedelolactone which can serve as a bioactive marker was estimated in karisalai juice and Karisalai Madakku Tailams (KMT1, KMT2 and KMT3). Results: Physico-chemical results of Karisalai Madakku Tailam (KMT1, KMT2 and KMT3) were comparable with the values of base sesame oil. But the content of wedelolactone in first processed oil KMT1 was estimated as 0.039% which increased to 0.0715% in KMT2 and further increased to 0.1048% in KMT3. Conclusion: Physico chemical parameters can be referred to examine the overall quality in the oil formulation at any of the madakku stage. HPTLC method would be a tool for the identification and quantification of wedelolactone in this medicated oil.

10aEclipta alba10aKaiyan tailam10aKarisalai10aPeroxide value10aWedelolactone1 aShanmugam, Murugammal1 aRajasekar, Subhasri1 aSekaran, Priyanka1 aRajasekaran, Hemavathy1 aRamachandran, Shakila uhttps://www.phcogres.com/article/2025/17/1/105530pres20251927