02170nas a2200217 4500008004100000245015900041210006900200260001700269300001200286490000700298520142400305653001501729653001201744653001101756653001401767653001601781100003001797700003201827700002901859856006401888 2024 eng d00aEvolution and Confirmation of UV Spectrophotometric Approach for Simultaneously Estimating Allicin and Azadirachta indica Extracts in Niosomal Formulation0 aEvolution and Confirmation of UV Spectrophotometric Approach for cOctober 2024 a923-9280 v163 a

Background: Generally, it is quite difficult to precisely tell the exact amount of drug in any formulation after formulating it using the active ingredient and other excipients. Aim: Based on the simultaneous equation method in niosome formulation, a straightforward, quick, accurate, error-free and cost-effective spectrophotometric approach for the simultaneous quantification of Allicin and Azadirachta indica has been devised. Materials and Methods: The method involved estimation using two wavelengths. Since the absorbance of Allicin and Azadirachta indica reaches its maximum at 273 and 257 nm, respectively, these wavelengths were used to measure the absorbance and to estimate their relative concentrations. Allicin and Azadirachta indica both follow Beer-Lambert's rule at concentrations between 10 and 50 μg/mL. Results: The strategy can be applied for the regular estimation of Allicin and Azadirachta indica niosome formulation using the simultaneous equations as the concentration in the formulation and recovery were quite good (>95%) and reliable. It was designed and verified in accordance with ICH criteria. Methanol was used as a solvent for the process. Conclusion: The method proved to be useful, simple and precise for quantifying the extracts.

10aAbsorbance10aExtract10aHerbal10aPrecision10aWavelengths1 aChaudhari, Shilpa, Pravin1 aNemmaniwar, Abhishek, Sunil1 aNikam, Sarika, Ankushrao uhttps://www.phcogres.com/article/2024/16/4/105530pres164105