03078nas a2200229 4500008004100000245012900041210006900170260001400239300001200253490000700265520228100272653002202553653002602575653003002601653002102631653000902652100002802661700003002689700003202719700003402751856006302785 2024 eng d00aSimplified Column Fractionation with DPPH Assay Guidance: Isolated a Potent Hepatoprotective Fraction from Cassia auriculata0 aSimplified Column Fractionation with DPPH Assay Guidance Isolate cJune 2024 a638-6510 v163 a

Background: Hepatoprotection is a critical area for research due to limitations of synthetic drugs and their side effects in the treatment of liver diseases. Natural compounds, particularly those derived from herbs with hepatoprotective therapeutical potential are serving as a best alternative for the same indication. Cassia auriculata, used traditionally in Indian folk medicine, has having a promising pharmacological activities, including hepatoprotective effects. Objectives: Present study aimed to evaluate using antioxidant activity to find out hepatoprotective compounds present in C. auriculata leaf extract and its solvent fractions through in vitro, in vivo experiments and LCMS analysis. Materials and Methods: The DPPH assay was employed to assess in vitro antioxidant activity, while cytotoxicity and hepatoprotective effects against H2O2-induced hepatotoxicity in HepG2 cell lines were investigated. Furthermore, the hepatoprotective activity of the most active Column fraction was evaluated against CCl4-induced hepatotoxicity in albino Wistar rats. LCMS analysis was utilized to identify bioactive compounds in the most active Column fraction and correlations between antioxidant activity and phenolic, tannin and flavonoid contents were also explored. Results: The findings of the present study suggest that most active Column fraction from C. auriculata possess significant antioxidant and hepatoprotective properties, making them promising candidates for further investigation as potential therapeutic agents for liver diseases. Conclusion: This study aims to bridge the gap in understanding the correlation between antioxidant and hepatoprotective potential of C. auriculata extract and its fractions through rigorous experimental investigations. Overall, the present study contributes to the growing body of evidence supporting the therapeutic potential of natural compounds in liver disease management, paving the way for further research and development in this field. Keywords: Cassia auriculata, Hepatoprotective activity, LCMS, HepG2 cell lines, Column chromatography.

10aCassia auriculata10aColumn chromatography10aHepatoprotective activity10aHepG2 cell lines10aLCMS1 aS, Tripathy, Amrutanand1 aRamesh, Manoj, Madanahall1 aShankar, Nischith, Sirawase1 aVenkatappa, Annegowda, Hardur uhttps://www.phcogres.com/article/2024/16/3/105530pres16375