02150nas a2200229 4500008004100000245014200041210007100183260001500254300001000269490000700279520136000286653002301646653002701669653003201696653001501728653001401743653002401757100001801781700002801799700003101827856006201858 2021 eng d00aPreparation of Diterpenes‑Enriched Extract from Croton stellatopilosus Ohba Leaves using Enzyme‑ and Ultrasonic‑Assisted Extraction0 aPreparation of Diterpenes‑Enriched Extract from Croton stellatop cApril 2021 a22-270 v133 a

Background: Croton stellatopilosus Ohba accumulates several diterpenes. Among them, plaunotol, a cytoprotective agent, has been prescribed to treat peptic ulcers. Objectives: We aimed to prepare the diterpene-enriched extract (DEE) using enzyme- and ultrasonic-assisted extractions. Materials and Methods: The type and amount of pectinase and cellulase and the condition of ultrasonic treatment were optimized. A combination of enzyme and ultrasonic treatments was performed. The high-performance liquid chromatography (HPLC) method was validated and used for the determination of plaunotol and plaunolide contents in the DEE. Results: A combination of cellulase (10 units) and ultrasonic (at 80% amplitude) treatments enhanced the yield of plaunotol significantly. Application of the crude extract on diaion HP-20 resin afforded the DEE. With the validated HPLC method, the DEE contained 8.81% ± 0.02% w/w of plaunotol and 8.40% ± 0.92% w/w of plaunolide. Conclusion: We established an efficient method to prepare DEE based on green extraction. This method showed about eightfold enhancement purification factor compared to the original crude extract and about 90% yield recovery.

10aCellulase-assisted10aCroton stellatopilosus10aDiterpenes-enriched extract10aPlaunolide10aPlaunotol10aUltrasonic-assisted1 aAung, Wai, Mi1 aLimsuwanchote, Supattra1 aWungsintaweekul, Juraithip uhttps://www.phcogres.com/article/2021/13/1/104103prpr9020