02602nas a2200301 4500008004100000245015900041210006900200260001600269300001200285490000700297520159000304653001301894653002601907653002601933653001701959653001701976653002201993100002102015700002302036700001802059700002602077700002502103700002602128700003302154700002102187700003102208856006102239 2019 eng d00aStudy of Antidiabetic Properties of Uvaria narum Leaf Extract through Glucose Uptake and Glucose Transporter 4 Expression Studies in 3T3L1 Cell Line Model0 aStudy of Antidiabetic Properties of Uvaria narum Leaf Extract th cAugust 2019 a304-3090 v113 a

Background: Uvaria narum (UN) is known to have antipyretic, antimicrobial, anti‑inflammatory and antimalarial properties. The antidiabetic properties of UN remains unexplored. The current study has been aimed at understanding the antidiabetic property of UN extract on an in vitro model using 3T3‑L1 cell line. Materials and Methods: Methanolic extract of UN was prepared, and its cytotoxic effect on 3T3‑L1 cells was assessed. Glucose uptake and glucose transporter 4 (GLUT4) translocation in 3T3‑L1 cell line on treatment with the extract was evaluated against a standard drug, metformin. α‑glucosidase and α‑amylase inhibition activities of the extract were also assayed with acarbose as the standard drug. Results: Treatment with UN extract had no cytotoxic effect on the cells. UN extract showed a good percentage inhibition of α‑amylase and α‑glucosidase activities. UN extract showed 71.31% inhibition and the control drug Acarbose exhibited 88.54% inhibition in α‑amylase activity. Furthermore, the extract showed 79.11% inhibition when Acarbose exhibited 87.35% inhibition in α‑glucosidase activity. IC50 values were also determined. Further, on treatment with the extract, 75.49% of 3T3‑L1 cells took up glucose and 70.67% had GLUT4 expression. Conclusion: UN extract enhances glucose uptake and GLUT4 expression, inhibits α‑amylase and α‑glucosidase activities, thereby demonstrating the antidiabetic properties in vitro.

10a3T3‑L110aAlucose transporter 410aAntidiabetic activity10aUvaria narum10aα‑amylase10aα‑glucosidase.1 aAlsawalha, Murad1 aJanardhana, P., B.1 aPadma, K., R.1 aReddy, Shiva, Shankar1 aAl-Subaie, Abeer, M.1 aBolla, Srinivasa, Rao1 aVeeraraghavan, Vishnu, Priya1 aJoseph, Joel, P.1 aMohan, Surapaneni, Krishna uhttps://www.phcogres.com/article/2019/11/3/104103prpr719