02348nas a2200217 4500008004100000245008000041210006900121260001700190300001200207490000700219520167600226653001401902653001701916653001901933653001801952100002601970700002201996700003002018700001802048856006402066 2023 eng d00aAcacia nilotica Pod Extract has an Anti-cancer Effect on the U937 Cell Line0 aAcacia nilotica Pod Extract has an Anticancer Effect on the U937 cOctober 2023 a771-7750 v153 a

Background: Acacia nilotica (A. nilotica), a tree that thrives in tropical regions, has been reported to have various medicinal uses and various pharmacological properties. Potential anti-cancer effects are one of the medical properties of parts of this tree. Objectives: To explore and evaluate the cytotoxic effect of A. nilotica, we used Acacia nilotica’s pod extract on U937 cell lines as an acute myeloid leukemia model. Materials and Methods: Microscopic analysis, flow cytometry, and WST-1 tests were performed on the U937 cell line after 72 hr of treatment with different concentrations of ethanol pod extract from Acacia nilotica (A. nilotica) to test for DNA cell cycle, cell viability and apoptosis, and to determine the IC50 of the drug. Results: Treatment with A. nilotica pod extract significantly induced cell death in U937-treated cells compared with untreated control cells. Microscopic analysis revealed morphological changes in dead and fragmented cells associated with a reduction in cell number. A reduction in cell viability with an increase of cells in the late apoptosis stage by flow cytometry indicated the cytotoxic effect of A. nilotica and accumulation of cells in the sub-G1 phase of the DNA cell cycle analysis. The IC50 was identified by the WST-1 assay. Conclusion: The findings of the present study suggest that A. nilotica may be employed as a natural anti-cancer agent in research on acute myeloid leukemia in humans.

10aApoptosis10acytotoxicity10aFlow cytometry10aNatural drugs1 aBakheit, Halla, Falih1 aTaurin, Sebastien1 aElamin, Elwaleed, Mohamed1 aBakhiet, Moiz uhttps://www.phcogres.com/article/2023/15/4/105530pres154081