02889nas a2200277 4500008004100000245014400041210006900185260001700254300001200271490000700283520197300290653001802263653001202281653002202293653002702315653001402342100002202356700002702378700003202405700003002437700002802467700000502495700001802500700002902518856006402547 2023 eng d00aEvaluation of Selectivity Index and Phytoconstituents Profile of Various Extracts from the Stem of Strychnos lucida R. Br. as Anti-malarial0 aEvaluation of Selectivity Index and Phytoconstituents Profile of cOctober 2023 a733-7500 v153 a

Introduction: Strychnos lucida R. Br. or Songga was empirically used as an anti-malarial and immunostimulant in the Tetun tribe, Indonesia. However, almost all plants from the genus Strychnos contain the alkaloid toxic compound strychnine. Objectives: The aims of this study are to determine in vitro/in vivo anti-malarial activity, to analyze the selectivity index, and to measure the phytoconstituents of various extracts (water, ethanol, ethyl acetate, n-hexane) from S. lucida stem. Materials and Methods: In vitro anti-malarial study was conducted against Plasmodium falciparum 3D7-chloroquine-sensitive, and in vitro cytotoxic was performed against Monkey kidney Vero cell. Plasmodium berghei ANKA-chloroquine sensitive infected malaria mice were used as a model for evaluation of in vivo anti-plasmodial. Phytoconstituents profile was determined using Thermo scientific LC-HRMS, and the m/z data was analyzed using Compound Discoverer software with mzCloud MS/MS Library. Results: Water (W), Ethanol (E), and Ethyl Acetate (EA) extract exhibited more potential in vitro/in vivo anti-malarial activity than n-hexane (H) extract (IC50 2.48±0.09; 2.45±0.02; 2.90±0.07; 7.64±0.30 μg/mL, respectively). The selectivity index of water, ethanol, ethyl acetate, and n-hexane extract are 211.47, 78.46, 249.62, and 32.39, respectively). According to the LC-HRMS profile, water and ethanol extract mainly contain alkaloid and phenolic compounds. Ethyl acetate and n-hexane mostly contain terpenoids and fatty acids. Conclusion: According to the result, we conclude that ethanol and water extract from S. lucida R. Br. display potential anti-malarial. Alkaloids and phenolic compounds probably have the most contribution to their anti-malarial activity.

10aAnti-malarial10aLC-HRMS10aSelectivity index10aStrycnos lucida R. Br.10aVero cell1 aKhasanah, Uswatun1 aShalas, Alvan, Febrian1 aIhsan, Bachtiar, Rifai Prat1 aWulandari, Lung, Ayu Asti1 aNurtyas, Nadela, Cintya1 a1 aAlipiani, Iin1 aSholiha, Ulfa, Malihatus uhttps://www.phcogres.com/article/2023/15/4/105530pres154078