02727nas a2200241 4500008004100000245012700041210006900168260001700237300001200254490000700266520195600273653001402229653001002243653001402253653001702267653002902284100002302313700001802336700002302354700001602377700002802393856006402421 2023 eng d00aPiper pedicellatum C. DC. Leaf Methanol Extract Inhibits Edema and Granuloma and Modulates Mediators and Cytokines in Rats0 aPiper pedicellatum C DC Leaf Methanol Extract Inhibits Edema and cOctober 2023 a679-6860 v153 a

Background: Piper pedicellatum is an ethnomedicinal plant used in various inflammatory conditions by many ethnic groups and exploration of its therapeutic potentials is imperative. Objective: This study was carried out to assess anti-inflammatory potential of the methanol extract of the plant leaves using in vivo experiments to validate the traditional uses. Materials and Methods: Carrageenan-induced paw edema and Cotton pellet-induced granuloma models were used to investigate into the anti-inflammatory potential of the plant extract in rats at 200 mg/kg and 400 mg/kg dose. Indomethacin (10 mg/kg) is used as reference standard. Serum levels of inflammatory markers and cytokines, hematological and serum hepatic enzyme indices were analyzed in granuloma-induced model. Results: The methanol extract suppressed Carrageenan-induced paw edema significantly at different time intervals. The highest percentage inhibition was observed at 3rd hr after Carrageenan injection, which was 42.67% and 49.33% for 200 mg/kg and 400 mg/kg dose of the extract respectively. In granuloma-induced model, the methanol extract at 200 mg/kg and 400 mg/kg dose inhibited exudate formation by 28.58% and 41.27%, respectively and inhibition of granuloma tissue was 32.45% and 43.71%, respectively along with significant decrement in serum C-reactive protein, Prostaglandin E2, Tumor necrosis factor-α, Interleukin-6 while increases in Interleukin-10 levels. Also, the results showed hematological and serum hepatic enzyme levels were rectified to near normal levels in treated rats. Conclusion: The outcome of the investigation reveals the potential of extract as possible anti-inflammatory agent, which can be develop into therapeutic agent or supplement and also necessitates further pharmacological studies on the plant.

10aCytokines10aEdema10aGranuloma10aInterleukins10aPiper pedicellatum C. DC1 aKalita, Dev, Jyoti1 aDeka, Kangkan1 aSailo, Ngurzampuii1 aDas, Sanjoy1 aKakoti, Bibhuti, Bhusan uhttps://www.phcogres.com/article/2023/15/4/105530pres154072