02566nas a2200313 4500008004100000024000700041245013100048210006900179260001400248300001200262490000700274520153400281653001601815653001501831653001701846653001901863653005501882653001001937100003101947700002301978700003402001700002202035700002902057700002802086700002002114700002702134700002702161856006402188 2023 eng d a1300aIn vitro Cytoprotective Activity Study of Cocoa (Theobroma cacao L.) Pod Husk Ethanolic Extract against Blue Light Irradiation0 aIn vitro Cytoprotective Activity Study of Cocoa Theobroma cacao  cJune 2023 a537-5430 v153 a

Background: Blue light exposure has been shown to induce ROS generation and subsequent inflammatory pathways that lead to cell death, in which antioxidants could counteract this effect. Although regarded as waste, cocoa (Theobroma cacao L.) Pod Husk (CPH) possesses a high polyphenolic contents, which could serve as antioxidants. Objectives: To characterize CPH ethanolic extract based on its antioxidant capacity and observe the cytoprotective ability of CPH in vitro upon blue light exposure. Materials and Methods: CPH ethanolic extract was characterized through total phenolic content, total flavonoid content, and three antioxidant assays, then treated on HaCaT cells, in which cell viability was measured through MTS assay. Results: CPH extract showed high phenolic and flavonoid contents and high antioxidant capacity through DPPH and FRAP assay. CPH extract started to exert cytotoxicity from concentrations of 400 μg/mL and above, while 100 μg/mL and above in AA. Furthermore, CPH extract showed significant cytoprotective effect at 50 μg/mL at 11.92 ± 0.83% cell viability increase, wherein AA failed to protect HaCaT cells at the same concentration at 15.79 ± 0.72% cell viability decrease. Conclusion: CPH could serve as an alternative as blue light protection agent as it was safe to be used at higher concentrations and was able to protect HaCaT cells from blue light irradiation better than AA.

10aAntioxidant10aBlue light10aCell Culture10aCocoa pod husk10aCocoa pod husk cytoprotectivity against bluelight.10aHaCaT1 aAdiyanto, Shakila, Angjaya1 aChriscensia, Erika1 aWibowo, Elizabeth, Chrestella1 aNathanael, Joshua1 aCrystalia, Audrey, Amira1 aPutra, Agus, Budiawan N1 aPriadi, Gunawan1 aFahrurrozi, Fahrurrozi1 aHartrianti, Pietradewi uhttps://www.phcogres.com/article/2023/15/3/105530pres153056