02569nas a2200241 4500008004100000245012000041210006900161260001400230300001200244490000700256520183800263653001502101653001902116653001802135653001502153653001902168653001802187100001802205700000502223700001902228700001702247856006302264 2022 eng d00aStudies on Wound Healing Potential of Polyherbal Formulation using in vitro and in vivo Assays in Swiss Albino Rats0 aStudies on Wound Healing Potential of Polyherbal Formulation usi cJuly 2022 a263-2680 v143 a

Background and Aim: Individual herbs, according to Ayurveda, are insufficient to provide the intended medicinal effect. When it is adjusted as a multiple herb composition in a certain ratio, it provides a greater medicinal impact with less toxicity. This investigation is an attempt to evaluate wound healing activity of poly herbal formulation along with in-vitro and in-vivo pharmacological activity on Swiss albino rats. Experimental Procedure: Full skin defects were made in the dorsal region of rats in order to examine wound healing. The activity of the polyherbal formulation was carried out by in-vitro (hydroxyproline, collagen and hexosamine) and in-vivo (excision and burn) assays along with histopathological study. Silver sulfadiazine was used as standard drug for the experimental work of this project while ketamine hydrochloride (20mg/kg intramuscular) was used as anesthize agent. The use of 2 μg/ml and 4 μg/ml formulations resulted in substantial mobilization of keratinocytes and fibroblasts at the injury site, respectively. In case of in vitro activity, effect of polyherbal formulation on biochemical measures such as hydroxyproline, collagen and hexosamine turnover were enhanced compared with untreated group. Results: Polyherbal formulation containing herbal extract was applied on wound area on day 0, 3rd, 6th, and 9th and was found to be more significant. Burn wound area on last day was observed 26.30 ± 0.5 while surgically produced wound area was 13.666± 0.8. Conclusion: Finally, on the basis of above results, we can conclude that polyherbal formulation may be a breakthrough for the treatment of wound healing in future.

10aBurn wound10aEpithelization10aExcised wound10aHexosamine10aScar formation10aWound healing1 aJogpal, Vikas1 a1 aSanduja, Mohit1 aPrakash, Ved uhttps://www.phcogres.com/article/2022/14/3/105530pres14338