02989nas a2200217 4500008004100000245015900041210006900200260001500269300001200284490000700296520216300303100002702466700002902493700003102522700002502553700003602578700003302614700002902647700003202676856006302708 2022 eng d00aSerjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice0 aSerjania marginata Casar Hydroalcoholic Extract Reduced Cytokine cApril 2022 a127-1340 v143 a

Objectives: This study investigated the antimycobacterial, anti-inflammatory and antihyperalgesic effects of hydroalcoholic extract from leaves of S. marginata (EESM) in in vitro and in vivo models. Methods and Results: EESM (0.98–1000 μg/ml) was evaluated in in vitro against Mycobacterium tuberculosis, M. bovis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis. The EESM oral administration (p.o.) (30, 100 and 300 mg/kg) and dexamethasone subcutaneous injection (s.c.) (1 mg/kg) were tested against the carrageenan-induced inflammatory paw edema and pleurisy in Swiss mice. The EESM (30 and 100 mg/kg, p.o.) and dexamethasone (1 mg/kg, s.c.) were tested against the CFA-induced paw inflammation and M. bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice. The minimum inhibitory concentration (MIC) of EESM in the presence of M. tuberculosis was 62.4 μg/ml. The values of MIC of EESM in the presence S. epidermidis, K. pneumoniae were 1000 μg/mL while EESM did not interfere against P. aeruginosa growth. EESM significantly inhibited paw edema/mechanical hyperalgesia in carrageenan induced paw inflammation and leukocytes migration/proteins exudation in carrageenan-induced pleurisy model. In the BCGinduced pleurisy model, the daily treatment for 7 days, with EESM inhibited the levels of IL-1β in blood and in pleural exudate. The EESM did not alter the mycobacterial growth in the cell culture from pleural lavage, spleen and liver samples collected from BCG-treated animals. The EESM significantly inhibited the persistent edema and mechanical hyperalgesia induced by CFA. Conclusion: This study confirms the EESM anti-inflammatory property and showed that EESM has high potency in inducing inhibition of mycobaterial growth and low potency or no effects in relation to other microorganisms.

Key words: Mycobacterium tuberculosis, Bacillus Calmette-Guerin (BCG), Cipó-timbó, Inflammation, Tuberculosis.

1 aLeitão, Maicon, Matos1 aRadai, Joyce, Alencar Sa1 aMacorini, Luis, Fernando B1 aFraga, Thiago, Leite1 aHeredia-Viira, Silvia, Cristina1 aCardoso, Claudia, Andrea Lim1 aArena, Arielle, Cristina1 aKassuya, Candida, Aparecida uhttps://www.phcogres.com/article/2022/14/2/105530pres14218