@article {326, title = {Effects of Ligusticum porteri (Osha) Root Extract on Human Promyelocytic Leukemia Cells}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {April 2017}, pages = {156-160}, type = {Original Article}, chapter = {156}, abstract = {

Background: Ligusticum porteri roots have been traditionally used in folk medicine, but the scientific basis is unclear. Objective: To investigate the cytotoxicity, antioxidant, and immunomodulatory effects of L. porteri root extract on human promyelocytic leukemia (HL-60) cells and H2O2-induced oxidative damaged HL-60 cells. Materials and Methods: HL-60 cells were incubated with different concentrations of root extract, and cells were harvested for viability assays on day 3 and 7. Cytokine levels (interferon-gamma [IFN-γ], interleukin-2 [IL-2], and interleukin-10 [IL-10]) and antioxidant indexes (malondialdehyde [MDA], reduced glutathione [GSH], superoxide dismutase [SOD], and catalase [CAT]) in H2O2-induced-stressed HL-60 were measured after 2 days. Results: The viability of HL-60 challenged with H2O2 declined by 42\% compared to unstressed cells. After 7 days of incubation with 200 or 400 μg/mL L. porteri, the viability of HL-60 cells was two-fold higher than the control. Stressed HL-60 cells treated with 100, 200, and 400 μg/mL L. porteri reduced the lipid peroxidation by 12\%{\textendash}13\%. We noted an increase in GSH levels, SOD and CAT activities in stressed HL-60 supplemented with 400 μg/mL root extract. Treatment with 400 μg/mL L. porteri significantly (P \< 0.05) increased IFN-γ and IL-2 in H2O2-challenged cells. Conclusion: Our data do not support the use of the extract as an antiproliferation and differentiation therapy for acute promyelocytic leukemia. The protective function of L. porteri root extract against oxidative stress could occur through increasing GSH and higher expression of antioxidant enzymes.

}, keywords = {Antioxidative, cytotoxicity, Human promyelocytic leukemia cells, Immune-modulatory, Ligusticum porteri}, doi = {10.4103/0974-8490.204641}, author = {Khanh Nguyen and Jean Sparks and Felix Omoruyi} } @article {684, title = {Antihyperglycemic and Antioxidative Effects of the Hydro-methanolic Extract of the Seeds of Caesalpinia Bonduc on Streptozotocin-induced Diabetes in Male Albino Rats}, journal = {Pharmacognosy Research}, volume = {4}, year = {2012}, month = {december,2011}, pages = {57-62}, type = {Original Article}, chapter = {57}, abstract = {

Background: No satisfactory effective treatment is available yet to cure diabetes mellitus. Though, synthetic drugs are used but there are several drawbacks. The attributed antihyperglycemic effects of many traditional plants are due to their ability for the management of diabetes mellitus. Materials and Methods: A hydromethanolic extract was administered orally at a dose of 250 mg/kg of body weight per day for 21 days. Its effects on the fasting blood glucose (FBG) level, activities of key carbohydrate metabolic enzymes like hexokinase, glucose-6-phosphatase, and glucose-6-phosphate dehydrogenase, and antioxidant enzymes like catalase and superoxide dismutase along with the effect on the lipid peroxidation level in hepatic tissues were measured. Glycogen levels were also assessed in hepatic and skeletal muscles and some toxicity parameters, such as serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and alkaline phosphates activities were measured. Results: Treatment of the hydromethanolic extract of the seeds of Caesalpinia bonduc resulted in a significant (P \< 0.05) recovery in the activities of carbohydrate metabolic enzymes along with correction in FBG and glycogen levels as compared with the untreated diabetic group. The extract also resulted in a significant (P \< 0.05) recovery in the activities of toxicity assessment enzyme parameters. Activities of antioxidant enzymes like catalase and superoxide dismutase along with the lipid peroxidation levels were also recovered significantly (P \< 0.05) after the treatment of the extract. The corrective effects produced by the extract were compared with the standard antidiabetic drug, glibenclamide. Conclusion: Our findings provide that the extract shows possible antihyperglycemic and antioxidative activities.

}, keywords = {Antihyperglycemic, Antioxidative, C. bonduc, Streptozotocin}, doi = {10.4103/0974-8490.91044}, author = {Kishalay Jana and Kausik Chatterjee and Kazi Monjur Ali and Debasis De and Tushar Kanti Bera and Debidas Ghosh} }