@article {1115, title = {Simultaneous Quantitative Determination of Six Triterpenoid Saponins in Ardisia japonica Collected from Different Regions of China through LC-ESI-MS}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {December 2022}, pages = {138-144}, type = {Original Article}, chapter = {138}, abstract = {

Background: The whole plants of Ardisia japonica (Myrsinaceae) are used in traditional Chinese medicine for the treatment of phlegm, cough, jaundice, edema, and bruise, and triterpenoid saponins are the main bioactive components. However, triterpenoid saponins from this plant have not yet been simultaneously quantitatively determined and reported. Objectives: The present study aimed to establish a fast, simple, and reliable LC-ESI-MS analysis method for simultaneous quantization of the major triterpenoid saponins in A. japonica for the first time. Materials and Methods: Six major triterpenoid saponins from the plant species of Ardisia were selected as the reference compounds. Five batches of A. japonica were gathered from five distinct regions of P. R. China. Quantitative analysis was developed and validated by LC-ESI-MS in SIM mode. The separation conditions were optimized on a RP C18 Column with ACN and 0.1\% FA (v/v) at a flow rate of 0.2 ml/min and column temperature of 35{\textdegree}C. Results: The validation investigation found that the analysis method was accurate, recoverable, and sensitive for quantification of the six triterpenoid saponins. All of the calibration curves revealed acceptable linear regression (R2\>0.9975). The contents of six triterpenoid saponins in A. japonica from five regions varied considerably. Conclusion: The present study established a highly efficient analytical technique to quantify the triterpenoid saponins in A. japonica, and it could be further applied in the quality control of A. japonica.

}, keywords = {Ardisia japonica, LC-MS, Quantitative analysis, Triterpenoid saponin}, doi = {10.5530/097484900260}, author = {Ling Ma and Mi Zhang and Kouharu Otsuki and Yan Lei and Takashi Kikuchi and Yinghua Wang and Wei Li} } @article {318, title = {Pharmacognostic Specification, Chlorogenic Acid Content, and In vitro Antioxidant Activities of Lonicera japonica Flowering Bud}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {April 2017}, pages = {128-132}, type = {Original Article}, chapter = {128}, abstract = {

Background: Lonicera japonica Thunb. or Japanese Honeysuckle has been widely used in traditional medicine for antipyretic. Objective: To establish the pharmacognostic specification of L. japonica flowering bud in Thailand and to determine its chlorogenic acid content and in vitro antioxidant activities. Materials and Methods: Dried L. japonica flowering bud from 15 various herbal drugstores throughout Thailand were investigated for pharmacognostic specification. Their chlorogenic acid contents were quantitatively analyzed by thin layer chromatography (TLC) densitometry with winCATS software. The mobile phase for TLC development consisted of ethyl acetate: formic acid: acetic acid: water (10:1.1:1.1:2.6). Antioxidant activities were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric ion reducing antioxidant power assay, nitric oxide scavenging assay, and β-carotene bleaching assays. Results: Qualified L. japonica flowering bud in Thailand was presented that the contents of loss on drying, total ash, acid-insoluble ash, and water should not be \>10.11\%, 6.59\%, 1.14\%, and 10.82\% by weight, respectively. The ethanol and water soluble extractive values should not be \<16.46\% and 28.88\% by weight, respectively. Chlorogenic acid content in L. japonica flowering bud was found to be 2.24 {\textpm} 0.50 g/100 g of crude drug. L. japonica flowering bud showed DPPH and nitric oxide scavenging activities as well as reducing power property. Conclusion: This pharmacognostic specification with special reference to the chlorogenic acid content can be used for quality control of L. japonica flowering bud in Thailand. The potential antioxidant of this crude drug was demonstrated in vitro.

}, keywords = {Antioxidant activity, Chlorogenic acid, Lonicera japonica Thunb., Pharmacognostic specification, Quantitative analysis, Thin layer chromatography-densitometry}, doi = {10.4103/pr.pr_117_16}, author = {Chayanon Chaowuttikul and Chanida Palanuvej and Nijsiri Ruangrungsi} }