@article {301, title = {Gas Chromatography-Mass Spectrometric Determination of Components of Leaves of Aegle marmelos and Psidium guajava and Seeds of Nigella sativa and Correlation with In vitro Antioxidant Activity}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {April 2018}, pages = {230-235}, type = {Short Communication}, chapter = {230}, abstract = {

Objective: The aim of this study is to analyze the volatile components of the leaves of Aegle marmelos, Psidium guajava, and seeds of Nigella sativa, using gas chromatography-mass spectrometric (GC-MS) and correlate with their in vitro antioxidant activity. Materials and Methods: Plant material was analyzed for heavy metal content (HMC), pesticide residue (PR), and the presence of microbes/ fungi. Following hydrodistillation, the volatile components (AM, PG, and NS) were subjected to GC-MS analysis and in vitro antioxidant assay (2,2-diphenyl-1-picrylhydrazyl). Results: The plant material passed the test for HMC, PR, microbial, and fungal contamination. Using GC-MS, the number of compounds identified in AM, PG, and NS were 62, 46, and 58, respectively. The antioxidant activity of AM, PG, and NS was 98.538\%, 98.955\%, and 97.755\%, respectively. Conclusion: GC-MS-based methods can be successfully utilized for phytochemical profiling and standardization of plant material.

}, keywords = {Aegle marmelos, Antioxidant activity, Gas chromatography-mass spectrometric, Nigella sativa, Psidium guajava, Volatile compounds}, doi = {10.4103/pr.pr_93_17}, author = {C. V. Jayachandran Nair and Sayeed Ahamad and Washim Khan and Varisha Anjum and Rajani Mathur} }