@article {280, title = {Growth Inhibition and Morphological Alteration of Fusarium sporotrichioides by Mentha piperita Essential Oil}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {February 2017}, pages = {74-79}, type = {Original Article}, chapter = {74}, abstract = {

Objective: The aim of this study is to determine the phytochemical composition, antifungal activity of Mentha piperita essential oil (MPE) against Fusarium sporotrichioides. Methods: The phytochemical composition was conducted by gas chromatography mass spectrometry (GC MS) analysis and mycelia growth inhibition was determined by minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), the morphological characterization was observed by scanning electron microscopy. Finally, the membrane permeability was determined by the release of extracellular constituents, pH, and total lipid content. Result: In GC MS analysis, 22 metabolites were identified such as menthol, l menthone, pulegone, piperitone, caryophyllene, menthol acetate, etc. The antifungal activity against targeted pathogen, with MIC and MFC 500 μg/mL and 1000 μg/mL, respectively. The MPE altered the morphology of F. sporotrichoides hyphae with the loss of cytoplasm content and contorted the mycelia. The increasing concentration of MPE showed increase in membrane permeability of F. sporotrichoides as evidenced by the release of extracellular constituents and pH with the disruption of cell membrane indicating decrease in lipid content of F. sporotrichoides. Conclusion: The observed results showed that MPE exhibited promising new antifungal agent against Fusarium sporotrichioides

}, keywords = {Fusarium sporotrichioides, Gas chromatography-mass spectrometryGC-MS, Mentha piperita essential oil, Scanning electron microscope}, doi = {10.4103/0974-8490.199771}, author = {P Rachitha and K Krupashree and GV Jayashree and Natarajan Gopalan and Farhath Khanum and K Krupashree and GV Jayashree and Natarajan Gopalan and Farhath Khanum} } @article {455, title = {Extraction and Elemental Analysis of Coleus forskohlii Extract}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June,2015}, pages = {237-241}, type = {Original Article}, chapter = {237}, abstract = {

Background: Coleus forskohlii Willd. is a popular traditional medicine used since ancient times for treatment of heart diseases, abdominal colic and respiratory disorders. Objectives: The aim of this study was to characterize the root extract of the medicinal plant Coleus forskohlii. Materials and Methods: Dry roots of C. forskohlii were used to extract Forskolin using toluene as a solvent. Thus, obtained extract of C. forskohlii was standardized to 30\% and used for further studies. Results: The physical properties of the extract were analyzed through scanning electron microscopy analysis, while the characterization of root extract through X-ray diffraction (XRD) and element analysis. The morphological feature of the C. forskohlii extract showed a flake like structure and the XRD showed sulfur trioxide (SO3) and trimer of sulfur trioxide (S3O9). Through element analysis, elements such as carbon, oxygen, magnesium, aluminum, silicon, phosphorous, and sulfur were identified. Carbon showed the highest weight of 75.49\% in comparison to all other elements.

}, keywords = {Coleus forskohlii, element analysis, Physical properties, Scanning electron microscope, X-Ray diffraction.}, doi = {10.4103/0974-8490.157966}, author = {Haritha Kanne and Narayan Pandurang Burte and V. Prasanna, Ravi Gujjula} }