@article {1014, title = {GC-MS Analysis and Thrombolytic Property of Methanolic Leaf Extracts of Terminalia pallida Brandis against Carrageenan Instigated Tail Thrombosis Model in Mice}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {January 2021}, pages = {53-60}, type = {Research Article}, chapter = {53}, abstract = {

Background: Several plants of Terminalia species are being reported as medicinally useful. Terminalia pallida Brandis is one of the plants of the Combretaceae family, which constitutes several pharmacologically active substances. Present work explored leaf extract{\textquoteright}s thrombolytic property, which could serve as the superior choice for currently using drugs. Objectives: The present study primarily focused on investigating the phyto-constituents such as phenolic and flavonoid compounds in methanolic leaf extract followed by GC-MS analysis and determining the in vitro thrombin inhibition and in vivo thrombolysis activity. Materials and Methods: Swiss albino mice (total = 36) were randomly distributed into six groups (n=6). Thrombosis was instigated by injecting 40 μL of 1\% carrageenan (Type-I) via the subplantar region of the right hind paw of each mouse. The plant extract was screened for its in vitro thrombin inhibitory potency. In animal studies, the size of the blood clots in mouse tails was recorded by administering the plant extracts at 100, 200 and 300 mg/kg body weight for every 24, 48 and 72 hr, respectively. Results: A dose of 200 and 300 mg/kg of the extract showed significant thrombolytic activity at 24, 48, and 72 hr (p\< 0.001) in a concentration-dependent manner when correlated with the control group. A reduction in the length of blood clots (p\< 0.01) was observed at 48 and 72 hr. In acute oral toxicity study, administration of leaf extract showed no mortality and no significant behavioral changes up to 2000 mg/kg dose. The GC-MS analysis explored the occurrence of about 16 eluted compounds; among these, few have been reported as medicinally useful, which accounts for the therapeutic importance of the plant. Conclusion: In conclusion, the methanolic extract of T. pallida Brandis leaves at 200, and 300 mg/kg showed significant inhibition of the Thrombosis in carrageenan instigated model of mice and in vitro thrombin activity.

}, keywords = {Carrageenan, Heparin, Quercetin., Terminallia pallida Brandis, Thrombosis}, doi = {10.5530/pres.14.1.9}, url = {https://phcogres.com/fulltext/v14i1/105530pres1419.html}, author = {Sarvan Kumar Guguloth and Narender Malothu and Prasanth DSNBK} } @article {232, title = {Evaluation of Antithrombotic Activities of Solanum xanthocarpum and Tinospora cordifolia}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {February 2018}, pages = {98-103}, type = {Original Article}, chapter = {98}, abstract = {

Background: Solanum xanthocarpum and Tinospora cordifolia have been reported to exhibit anti-inflammatory, antiarthritic, antioxidant, antiallergic, and hepatoprotective activities. The origins of many of the currently available antithrombotic treatments are from natural products and natural sources. Objective: To investigate the antithrombotic activities of methanolic leaf extracts of S. xanthocarpum (SXME) and T. cordifolia (TCME). Materials and Methods: Antithrombotic activities were assessed by thrombin inhibition assay, thrombin generation assay, platelet adhesion assay on collagen-coated surface, and platelet PAC1-FITC binding by flow cytometry. Results: SXME significantly inhibited thrombin activity at 5{\textendash}20 mg/ml concentrations, whereas TCME inhibited thrombin activity at 500 {\textmu}g/ml{\textendash}5 mg/ml concentrations. Further, SXME inhibited thrombin generation at 2{\textendash}20 mg/ml concentrations, whereas TCME exhibited significant inhibition at 200 {\textmu}g/ml, suggesting that TCME has higher efficacy as compared to SXME. Moreover, SXME did not inhibit platelet adhesion on collagen-coated surface, whereas TCME inhibited platelet adhesion on collagen-coated surface at 5 mg/ml. Indomethacin showed significant inhibition in platelet adhesion at 300 {\textmu}M. Further, SXME inhibited thrombin-induced platelet activation (PAC1-FITC binding) significantly at 1 mg/ml by about 80\%, whereas TCME inhibited thrombin-induced platelet activation (PAC1-FITC binding) by about 40\% at 1 mg/ml. Conclusion: These results strongly suggested that SXME and TCME possess antithrombotic activities. However, further studies are essential to find out the active constituent responsible for antithrombotic effect.

}, keywords = {Collagen, Platelet, Solanum xanthocarpum, Thrombin, Thrombosis, Tinospora cordifolia}, doi = {10.4103/pr.pr_80_17}, author = {Onila Lugun and Sobharani Bhoi and Priyanka Kujur and D. Vinay Kumar and William R. Surin} }