@article {215, title = {Cytotoxic Compounds from Wrightia pubescens (R.Br.)}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {February 2018}, pages = {9-15}, type = {Original Article}, chapter = {9}, abstract = {

Background: Mixtures of ursolic acid (1) and oleanolic acid (2) (1:1 and 1:2), oleanolic acid (2), squalene (3), chlorophyll a (4), wrightiadione (5), and α-amyrin acetate (6) were isolated from the dichloromethane (CH2Cl2) extracts of the leaves and twigs of Wrightia pubescens (R.Br.). Objectives: To test for the cytotoxicity potentials of 1{\textendash}6. Materials and Methods: The antiproliferative activities of 1{\textendash}6 against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast neonatal (HDFn), were evaluated using the PrestoBlue{\textregistered} cell viability assay. Results: Compounds 4, 1 and 2 (1:2), 2, 1 and 2 (1:1), and 5 exhibited the most cytotoxic effects against HT-29 with half maximal inhibitory concentration (IC50) values of 0.68, 0.74, 0.89, 1.70, and 4.07 μg/mL, respectively. Comparing 2 with its 1:1 mixture with 1 (IC50 = 1.70 and 7.18 μg/mL for HT-29 and HCT-116, respectively) and 1:2 mixture with 1 (0.74 and 3.46 μg/mL for HT-29 and HCT-116, respectively), 2 also showed strong cytotoxic potential against HT-29 and HCT-116 (0.89 and 2.33 μg/mL, respectively). Unlike the mixtures which exhibited low effects on MCF-7 (IC50 = 20.75 and 30.06 μg/mL for 1:1 and 1:2, respectively), 2 showed moderate activity against MCF-7 (10.99 μg/mL). Compound 6 showed the highest cytotoxicity against HCT-116 (IC50 = 4.07 μg/mL). Conclusion: Mixtures of 1 and 2 (1:1 and 1:2), 2, 3, 4, 5, and 6 from the CH2Cl2 extracts of the leaves and twigs of W. pubescens (R.Br.) exhibited varying cytotoxic activities. All the compounds except 6 exhibited the strongest cytotoxic effects against HT-29. On the other hand, 6 was most cytotoxic against HCT-116. Overall, the toxicities of 1{\textendash}6 were highest against HT-29, followed by HCT-116 and MCF-7. All the compounds showed varying activities against HDFn (IC50 \<30 μg/mL).

}, keywords = {Apocynaceae, Chlorophyll a, cytotoxicity, Half maximal inhibitory concentration, HCT-116, HDFn, HT-29, MCF-7, Oleanolic acid, PrestoBlue{\textregistered} cell viability assay, Squalene, Ursolic acid, Wrightia pubescens R. Brown, Wrightiadione, α-amyrin acetate}, doi = {10.4103/pr.pr_45_17}, author = {Mariquit M. De Los Reyes and Glenn G. Oyong and Vincent Antonio S. Ng and Consolacion Y. Ragasa} } @article {536, title = {Antiproliferative evaluation of terpenoids and terpenoid coumarins from Ferulago macrocarpa (Fenzl) Boiss. fruits}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {October 2015}, pages = {322-328}, type = {Original Article}, chapter = {322}, abstract = {

Background: Ferulago macrocarpa is a plant used as flavoring agent and protectant in the food industry and as a folk medicinal plant in Iran with no available information on its chemical identity. Ferulago spp. showed to contain biologically terpenoids and coumarins. Objectives: The objective was to isolate and characterize terpenoids and coumarins from the acetone extract of F. macrocarpa fruits and to evaluate their antiproliferative effects on several cell lines. Materials and Methods: A series of normal and reverse phase gravity and high-performance liquid chromatography analyses were used to purify constituents. Compounds 1{\textendash}5 and 7 were evaluated for their cytotoxic effects on MCF-7, HT-29 and H-1299 cell lines. Results: Six compounds including bornyl acetate (1), 1,10-di-epi-cubenol (2), stigmasterol (3) and three coumarins grandivittin (4), prantschimgin (5) and 4{\textquotedblright}-hydroxygrandivittin (7) along with mixtures of feruloyl derivatives (6a-6c) have been purified. Their structures were established by spectroscopic methods including nuclear magnetic resonance and MS analyses. Compound 2 showed moderate cytotoxicity effect with IC50 values of 5.0 and 6.7 μM on MCF-7 and HT-29, respectively. Conclusion: 1,10-di-epi-Cubenol could be considered as a potential proliferation inhibitor of MCF-7 and HT-29 cell lines.

}, keywords = {1, 10-di-epi-Cubenol, Bornyl acetate, Grandivittin, HT-29, MCF-7, Prantschimgin.}, doi = {10.4103/0974-8490.158437}, author = {Seyed-Ebrahim Sajjadi and Maryam Jamali and Yalda Shokoohinia and Gisya Abdi and Behzad Shahbazi and Ali Fattahi} }