@article {185, title = {Gas Chromatography-High-Resolution Mass Spectrometry Elucidation and in vitro Cell Line Studies (Sulforhodamine B) on Niosomal Gel of Benincasa hispida}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {January 2021}, pages = {342-351}, type = {Original Article}, chapter = {342}, abstract = {

Background: The Cucurbitaceae family has been known for its presence of phytoconstituents, namely, lupeol, β-sitosterol, terpenoids, phenols, and proteins with therapeutic potential. Objectives: The research paper tries to focus on major therapeutic field of people demographic area of type of cancer. Soxhlet extraction of Benincasa hispida (BH) was carried out and crude extract was converted to powder. Niosomal gel was formulated after the optimization of drug concentration from the extract of Niosomal solution. Solution with maximum entrapment was selected for the preparation of gel. Gel was prepared by using different Carbopol grades and with comparison of different parameters. Gel was studied for gas chromatography-high-resolution mass spectrometry (GC-HRMS) evaluation for check toxicity. Biologic activity was analyzed by SRB (Sulforhodamine B) assay with in vitro cell lines (human oral cancer cell line KB and Mus Musculus skin melanoma B16-F10). Results: Solution which was clear and white was selected for gel development. Carbopol 934 (1{\textquoteright}) as gelling agent showed good formulation properties. It was non-gritty, easily spreadable, and washable. The gel with maximum entrapment efficiency was evaluated further. GC-HRMS revealed 1.2-ethyl 2-Hexen-1-ol; 1,8-(3-Octyl-2-oxiranyl)-1-octanol; Dodecanoic acid, 2-penten-1-yl ester; Cholestane, 4, 5-epoxy-,(4α,5α); Cyclopentaneundecanoic acid; 17-octadecynoic acid; Octadecanoic acid, methyl ester; Methyl oleate; Tridecanoic acid, methyl ester; and Silane, dimethyl (2,3,6, tricholrophenoxy) heptadicycloxy. The selected cell lines did not show satisfactory activity at different concentrations in SRB assay. Conclusion: This research paper tried to witness the probable potential of BH for cancer with analytical base. Future studies may be undertaken at various stages and levels to further explore the potential of these families of plants.

}, keywords = {Benincasa hispida, Gas chromatography-high-resolution mass spectrometry, Niosomal gel, Oral cancer, Sulforhodamine B}, doi = {10.4103/pr.pr_37_20}, author = {Gaurav Mahesh Doshi and Akanksha Dilip Badgujar and Ishan Sudhir Mathurvaishya} } @article {518, title = {Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {281-286}, type = {Original Article}, chapter = {281}, abstract = {

Background: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. Materials and Methods: Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. Results: Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. Conclusions: Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.

}, keywords = {Benincasa hispida, Carissa congesta, Liquid chromatography, Mass spectroscopy, Polyalthia longifolia, Quercetin, Rutin}, doi = {10.4103/0974-8490.186578}, author = {Gaurav M. Doshi and Vivek V. Nalawade and Aaditi S. Mukadam and Pratip K. Chaskar and Sandeep P. Zine and Rakesh R. Somani and Hemant D. Une} } @article {385, title = {Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {December 2015}, pages = {37-42}, type = {Original Article}, chapter = {37}, abstract = {

Objective: In Indian Ayurvedic system, Benincasa hispida (BH) and Carissa congesta (CC) are well-known plants used for major and minor ailments. BH has been regarded as Kushmanda, whereas CC has been used in immune-related disorders of the human system. Quercetin and rutin identified from the vast plethora of plant extracts have proved to possess ethnopharmacological relevance. Materials and Methods: In present studies, we have determined quercetin and rutin in terms of percentage in BH seeds and CC roots by high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin and rutin by HPTLC and HPLC. Results: HPTLC showed quercetin as 44.60, 27.13\% and rutin as 32.00, 36.31\% w/w, whereas HPLC revealed quercetin as 34.00, 35.00\% and rutin as 21.99, 45.03\% w/v in BH and CC extracts, respectively. Conclusion: The BH and CC extracts have elucidated peaks that were corresponding with standard peaks on undertaking chromatographic studies.

}, keywords = {Benincasa hispida, Carissa congesta, High-performance liquid chromatography, high-performance thin layer chromatography, Quercetin, Rutin}, doi = {10.4103/0974-8490.171098}, author = {Gaurav Mahesh Doshi and Hemant Devidas Une} } @article {471, title = {Structural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by gas chromatography: Mass spectroscopy}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June,2015}, pages = {282-293}, type = {Original Article}, chapter = {282}, abstract = {

Background: Benincasa hispida (BH) and Carissa congesta (CC) are regarded as ethnopharmacological imperative plants in Asian countries. Objectives: Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in the BH and CC extracts. The presence of lupeol has been reported previously by us using high-performance thin-layer chromatography and high-performance liquid chromatography. Materials and Methods: Present research studies encompasses identification of chemical constituents in BH seeds and CC roots petroleum ether extracts by hyphenated technique such as gas chromatography-mass spectroscopy (MS) which when coupled gives a clear insight of constituents. Results: The components were identified by matching mass spectra with MS libraries. There were 13 and 10 different compounds analyzed from CC and BH, respectively. The components present were Pentanoic acid, 5-hydroxy, 2,4-butylphenyl; n-Hexadecanoic acid (Palmitic acid); Sulfurous acid, 2-ethylhexylhepatdecyl ester; n-Tridecane; 6-methyltridecane; (9E, 12E)-9,12-Octadecadienyl chloride, Hexadecanoic acid, 3-(trimethylsilyl)-oxy] propyl ester; 9,12-Octadecadenoic acid, 2 hydroxy-1-(hyroxymethylethyl) ester; 9,12-Octadecadienoic acid, 2,3 dihydroxypropyl ester; n-Propyl-9,12-Octadecadienoate, Lupeol; Taraxasterol; 6a, 14a-Methanopicene, perhydro-12,4a, 61a, 9,9,12a-hepatmethyl-10-hydoxy and 9-Octadecene; 2-Isoprpenyl-5-methyl-6-hepten-1-ol; n-Hexadecanoic acid, 2-hyroxy-1-(hydroxymethyl) ethyl ether; Butyl-9,12-Octadecadieonate; Friedoolean-8-en-3-one; friedours-7-en-3-one; 13,27-Cyclosuran-3-one; Stigmaste-7,25-dien-3-ol (3β, 5α); Stigmasta-7,16-dien-3-ol; chrondrillasterol in BH seeds and CC roots extracts respectively. Conclusion: Eluted components from the extracts could provide further researchers to work with various pharmacological activities related models and studies.

}, keywords = {25-dien-3-ol., Apocynaceae, Benincasa hispida, Carissa congesta, Chrondrillasterol, Cururbitaceae, Gas chromatography, Lupeol, Mass spectroscopy, Stigmaste-7}, doi = {10.4103/0974-8490.157179}, author = {Gaurav M. Doshi and Vivek V. Nalawade1 and Aaditi S. Mukadam and Pratip K. Chaskar and Sandeep P. Zine and Rakesh R. Somani and Hemant D. Une} }