@article {1338, title = {Standardization of Karisalai Madakku Tailam and Quantification of Wedelolactone by HPTLC}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {78-84}, type = {Original Article}, chapter = {78}, abstract = {

Background: Herbal oils also known as Medicated oils, are oils infused with medicinal properties of herbs herbs plants. Objectives: To prepare the herbal oil, Karisalai tailam using gingelly oil and Eclipta alba Linn. (Karisalai) further to continue the process of madakku for two more time and to investigate the physico-chemical parameters for any changes. Materials and Methods: The herbal oil was prepared as per literature. Acid, saponification, iodine, peroxide values, rancidity, refractive index were estimated for the raw gingelly oil, gingelly oil heated in parallel without adding Eclipta alba Linn. juice, Karisalai Madakku Tailam (KMT1), second Madakku Tailam (KMT2) and third madakku tailam (KMT3). The content of major coumestane derivative present in Eclipta alba Linn., ie., wedelolactone which can serve as a bioactive marker was estimated in karisalai juice and Karisalai Madakku Tailams (KMT1, KMT2 and KMT3). Results: Physico-chemical results of Karisalai Madakku Tailam (KMT1, KMT2 and KMT3) were comparable with the values of base sesame oil. But the content of wedelolactone in first processed oil KMT1 was estimated as 0.039\% which increased to 0.0715\% in KMT2 and further increased to 0.1048\% in KMT3. Conclusion: Physico chemical parameters can be referred to examine the overall quality in the oil formulation at any of the madakku stage. HPTLC method would be a tool for the identification and quantification of wedelolactone in this medicated oil.

}, keywords = {Eclipta alba, Kaiyan tailam, Karisalai, Peroxide value, Wedelolactone}, doi = {10.5530/pres.20251927}, author = {Murugammal Shanmugam and Subhasri Rajasekar and Priyanka Sekaran and Hemavathy Rajasekaran and Shakila Ramachandran} } @article {666, title = {Corrected and Republished: Comparative Botanical and Phytochemical Studies of Ambiguous Medicinal Plant Species of Wedelia and Eclipta (Fam. Asteraceae) Used in ASU Systems of Medicine with Special Reference to in-silico Screening of Hepatoprotective Pote}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {April 2021}, pages = {54-67}, type = {Original Article}, chapter = {54}, abstract = {

Post the publication, as there were multiple corrections that were missed earlier, the article is being corrected and republished.

Comparative Botanical and Phytochemical Studies of Ambiguous Medicinal Plant Species of\ Wedelia\ and Eclipta (Fam. Asteraceae) Used in ASU Systems of Medicine with Special Reference to\ in-silico\ Screening of Hepatoprotective Potential of Marker Wedelolactone with Acetaminophen Targets.\ 

Context:\ In traditional medicine,\ Kesaraja\ (Ayurveda) or\ Manjal karisali\ (Siddha) is effective for jaundice.\ Aim:\ Three species of Asteraceae need to be studied for their therapeutic superiority of their intended claim. They are\ Wedelia chinensis\ (Osbeck) Merr. Philipp J.,\ Wedelia trilobata\ (L.) Hitchc. and\ Eclipta prostrata\ (L.) L. (Asteraceae). The present study aimed to screen and characterize the potential species for therapeutic purpose.\ Materials and Methods:\ The whole plants,\ W. chinensis\ (Osbeck) Merr.\ W. trilobata\ (L.) Hitchc. and\ E. prostrata\ (L.) (Asteraceae) were collected and botanically identified. Preliminary phytochemical analysis and high-performance thin-layer chromatography finger printing with marker wedelolactone were done for the ethanolic extracts of these plants. Botanical and pharmacognostical diagnostic characters of the plants based on macro-morphological, micro-morphological and powder microscopical characterization were worked out. Comparative\ in-vitro\ antioxidant potential of ethanolic extracts of these plant species was carried out. Using ADMET SAR software, the pharmacokinetics of wedelolactone were predicted. Using Autodock 4.2 software, the binding energy of wedelolactone on targets of acetaminophen-induced hepatotoxicity namely PPAR-α, AMPK, JNK-1, EGFR, Nrf2, ALT, ALP, GGT, CAR, Frizzled receptor, FXR, ERK1, LXR, mitochondrial glutamate dehydrogenase, p53, mTOR C1, CYP1A2, CYP2E1, 5-lipoxygenase, thrombin, UCP1, GSK1, RXR and PXR was predicted.\ Results:\ All the three plant species were pharmacognostically and chemically different.\ W. chinensis\ was found to possess more antioxidant potential than the other two plants. The marker compound wedelolactone was not detected in\ W. trilobata.\ Wedelolactone passed the Lipinski{\textquoteright}s rule of five, and the docking analysis of wedelolactone confirmed high binding affinity toward PPAR-α, AMPK, Nrf2, CYP2E1, EGFR, JNK1, UCP-2, thrombin, 5-lipoxygenase, mTORC1, RXR, FXR, LXR, Frizzled receptor, GDH and Erk-1.\ Conclusion:\ Based on the above observations, we conclude that the presence of marker compound wedelolactone might have attributed the potency of\ W. chinensis\ and\ E. prostrata\ in counteracting acetaminophen toxicity when compared with\ W. trilobata.

}, keywords = {Binding energy, botanical standardization, chromatographic fingerprinting, Docking, Hepatoprotective, in silico prediction, Lipinski{\textquoteright}s rule, Siddha, Therapeutic superiority, Wedelolactone}, doi = {10.4103/pr.pr_11_21}, author = {C. Arunachalam and R. Arunadevi and S. Murugamma and N. Monika and R. Susila and K. N. Sunil Kumar} } @article {171, title = {Comparative Botanical and Phytochemical Studies of Ambiguous Medicinal Plant Species of Wedelia and Eclipta (Fam. Asteraceae) Used in ASU Systems of Medicine with Special Reference to in-silico Screening of Hepatoprotective Potential of Marker Wedelolacto}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {August 2020}, pages = {285-298}, type = {Original Article}, chapter = {285}, abstract = {

Context: In traditional medicine, Kesaraja (Ayurveda) or Manjal karisali (Siddha) is effective for jaundice. Aim: Three species of Asteraceae need to be studied for their therapeutic superiority of their intended claim. They are Wedelia chinensis (Osbeck) Merr. Philipp J., Wedelia trilobata (L.) Hitchc. and Eclipta prostrata (L.) L. (Asteraceae). The present study aimed to screen and characterize the potential species for therapeutic purpose. Materials and Methods: The whole plants, W. chinensis (Osbeck) Merr. Philipp J., W. trilobata (L.) Hitchc. and Eclipta prostrata (L.) (Asteraceae) were collected and botanically identified. Preliminary phytochemical analysis and high-performance thin-layer chromatography finger printing with marker wedelolactone were done for the ethanolic extracts of these plants. Botanical and pharmacognostical diagnostic characters of the plants based on macro-morphological, micro-morphological and powder microscopical characterization were worked out. Comparative in-vitro antioxidant potential of ethanolic extracts of these plant species was carried out. Using ADMET SAR software, the pharmacokinetics of wedelolactone were predicted. Using Autodock 4.2 software, the binding energy of wedelolactone on targets of acetaminophen-induced hepatotoxicity namely PPAR-α, AMPK, JNK-1, EGFR, Nrf2, ALT, ALP, GGT, CAR, Frizzled receptor, FXR, ERK1, LXR, mitochondrial glutamate dehydrogenase, p53, mTOR C1, CYP1A2, CYP2E1, 5-lipoxygenase, thrombin, UCP1, GSK1, RXR and PXR was predicted. Results: All the three plant species were pharmacognostically and chemically different. W. chinensis was found to possess more antioxidant potential than the other two plants. The marker compound wedelolactone was not detected in W. trilobata. Wedelolactone passed the Lipinski{\textquoteleft}s rule of five, and the docking analysis of wedelolactone confirmed high binding affinity toward PPAR-α, AMPK, Nrf2, CYP2E1, EGFR, JNK1, UCP-2, thrombin, 5-lipoxygenase, mTORC1, RXR, FXR, LXR, Frizzled receptor, GDH and Erk-1. Conclusion: Based on the above observations, we conclude that the presence of marker compound wedelolactone might have attributed the potency of W. chinensis and E. prostrata in counteracting acetaminophen toxicity when compared with W. trilobata.

}, keywords = {Antioxidant, Ayurveda, Botany, Docking, Eclipta prostrata, High-performance thin-layer chromatography, Macro-morphological, Micro-morphological, Phytochemical, Powder microscopy, Siddha, Wedelia chinensis, Wedelia trilobata, Wedelolactone}, doi = {10.4103/pr.pr_11_20}, author = {C Arunachalam and R Arunadevi and S Murugammal and N Monika and R Susila and S N Sunil Kumar} }